Protective Efect of Brain-derived Neurotrophic Factor Against the Ototoxicity ofPseudomonas aeruginosaExotoxin A
| Autor: | Manabu Maeta, Matti Anniko |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Virulence Factors Hearing Loss Sensorineural Bacterial Toxins Ear Middle Exotoxins Audiology Ototoxicity Internal medicine Evoked Potentials Auditory Brain Stem otorhinolaryngologic diseases medicine Animals Inner ear Auditory Fatigue Pitch Perception Cochlear Nerve Cochlea ADP Ribose Transferases Brain-derived neurotrophic factor Round window business.industry Brain-Derived Neurotrophic Factor Auditory Threshold General Medicine medicine.disease NG-Nitroarginine Methyl Ester Neuroprotective Agents Endocrinology medicine.anatomical_structure Round Window Ear Otorhinolaryngology Middle ear Audiometry Pure-Tone Sensorineural hearing loss sense organs business Auditory fatigue |
| Zdroj: | Acta Oto-Laryngologica. 123:14-19 |
| ISSN: | 1651-2251 0001-6489 |
| DOI: | 10.1080/0036554021000028090 |
| Popis: | OBJECTIVE: The protective effect of brain-derived neurotrophic factor (BDNF) against the ototoxicity resulting from exposure of Pseudomonas aeruginosa exotoxin A (PaExoA) to the middle ear was analyzed. The combined effect of BDNF and N(G)-nitro-L-arginine methyl ester (L-NAME) was also investigated. MATERIAL AND METHODS: Six groups of albino rats were instilled through the tympanic membrane into the round window niche with the following solutions: saline; PaExoA; BDNF; L-NAME; PaExoA + BDNF; and PaExoA + BDNF + L-NAME. Frequency-specific (2-31.5 kHz) auditory brainstem responses were used to obtain the hearing thresholds before and 2, 5 and 15 days after instillation. RESULTS: PaExoA penetrated from the middle ear into the cochlea, causing initially mixed hearing loss, followed by persistent sensorineural hearing loss. This impairment was blocked by BDNF at 6, 8 and 10 kHz on Day 2 and at 8 kHz on Day 5. L-NAME given in combination with BDNF did not show any additional protective effect. There were no significant differences in the thickness of the round window membrane between control ears and those in each instillation group. CONCLUSION: Our results suggest that BDNF may protect against cochlear damage caused by PaExoA in the middle turns of the ear. |
| Databáze: | OpenAIRE |
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