Culture conditions for arresting and stimulating the proliferation of a rainbow trout fibroblast cell line, RTG-2
| Autor: | A. Martinez, L. E. J. Lee, Niels C. Bols |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Confluency
Mitotic index Time Factors DNA synthesis Cell growth Trout Clinical Biochemistry Plant Science Cell Biology Anatomy Biology Fibroblasts Andrology medicine.anatomical_structure Cell culture Mitotic Figure medicine Animals Fibroblast Growth Substances Mitosis Cell Division Salmonidae Biotechnology Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology : journal of the Tissue Culture Association. 24(8) |
| ISSN: | 0883-8364 |
| Popis: | Conditions for arresting and stimulating the proliferation of the rainbow trout fibroblast cell line RTG-2 have been examined and the time course of events after stimulation determined. Quiescent populations were achieved in two ways. Cultures grown to confluency without a medium change for at least 7 d had fewer than 5% of the cells in S phase and few mitotic figures. Cultures deprived of serum, which could be done for up to 3 d without a loss in cell number, also achieved quiescence. After 3 d without serum, less than 1% of cells were in S phase and mitotic figures were infrequent. Addition to these cultures of fresh serum-containing medium brought about the synchronous entry of cells into S phase and mitosis. For cultures in which either the medium had been changed after 7 d without a change or serum-containing medium had been added after 3 d of serum deprivation, DNA synthesis increased after a lag period of 20 to 24 h, was pronounced between 30 and 45 h, and then declined. This was followed by a peak in the mitotic index. These protocols for arresting and subsequently stimulating RTG-2 proliferation should allow the G1-S transition to be studied in a representative of teleosts. |
| Databáze: | OpenAIRE |
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