Working with Auditory HEI-OC1 Cells
| Autor: | Federico Kalinec, Channy Park, Gilda M. Kalinec, Pru Thein |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Programmed cell death General Chemical Engineering Cellular differentiation Cell Drug Evaluation Preclinical Cell Culture Techniques non-linear capacitance General Biochemistry Genetics and Molecular Biology Cell Line Mice 03 medical and health sciences Issue 115 Hair Cells Auditory Autophagy medicine Animals Psychology Viability assay Prestin Auditory cell viability cell culture General Immunology and Microbiology biology Cell growth General Neuroscience patch-clamp Preclinical Cell biology Cellular Biology HEI-OC1 cells prestin motor function Hair Cells 030104 developmental biology medicine.anatomical_structure cell death Cell culture cell senescence biology.protein Drug Evaluation cytotoxicity Cognitive Sciences Generic health relevance Biochemistry and Cell Biology autophagy.FACS |
| Zdroj: | Kalinec, GM; Park, C; Thein, P; & Kalinec, F. (2016). Working with Auditory HEI-OC1 Cells. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (115). doi: 10.3791/54425. UCLA: Retrieved from: http://www.escholarship.org/uc/item/07t1c0x8 Journal of visualized experiments : JoVE, iss 115 |
| DOI: | 10.3791/54425. |
| Popis: | HEI-OC1 is one of the few mouse auditory cell lines available for research purposes. Originally proposed as an in vitro system for screening of ototoxic drugs, these cells have been used to investigate drug-activated apoptotic pathways, autophagy, senescence, mechanism of cell protection, inflammatory responses, cell differentiation, genetic and epigenetic effects of pharmacological drugs, effects of hypoxia, oxidative and endoplasmic reticulum stress, and expression of molecular channels and receptors. Among other several important markers of cochlear hair cells, HEI-OC1 cells endogenously express prestin, the paradigmatic motor protein of outer hair cells. Thus, they can be very useful to elucidate novel functional aspects of this important auditory protein. HEI-OC1 cells are very robust, and their culture usually does not present big complications. However, they require some special conditions such as avoiding the use of common anti-bacterial cocktails containing streptomycin or other antibiotics as well as incubation at 33 °C to stimulate cell proliferation and incubation at 39 °C to trigger cell differentiation. Here, we describe how to culture HEI-OC1 cells and how to use them in some typical assays, such as cell proliferation, viability, death, autophagy and senescence, as well as how to perform patch-clamp and non-linear capacitance measurements. |
| Databáze: | OpenAIRE |
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