Effects of microwave on extracellular vesicles and microRNA in milk
| Autor: | Siran Yu, Ping Li, Minjing Xu, Zhehao Zhao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Total rna Extracellular vesicles Cow milk Extracellular Vesicles 03 medical and health sciences chemistry.chemical_compound fluids and secretions 0302 clinical medicine Dynamic light scattering Mirna expression microRNA Genetics Animals Food science Microwaves Acridine orange food and beverages Staining MicroRNAs Milk 030104 developmental biology chemistry 030220 oncology & carcinogenesis Animal Science and Zoology Food Science |
| Zdroj: | Journal of Dairy Science. 101:2932-2940 |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.2016-12021 |
| Popis: | This study demonstrates the effects of microwaves on the microRNA (miRNA) content of milk and milk extracellular vesicles (EV). We determined the miRNA concentration in milk subjected to different treatments using real-time PCR and a spectrophotometer. The miRNA expression and total RNA content of the microwaved milk samples were lower when compared with untreated milk. We measured the microstructure and the size distribution by scanning electron microscopy and dynamic light scattering to verify the loss of miRNA in microwaved milk due to damage to the EV. The results revealed that 2 different-sized EV were present and had an average size of 147.50 and 22.14 nm, respectively. Furthermore, acridine orange staining showed that the total RNA content in microwaved milk EV was lower than that in cow milk. These results suggest that EV may confer the protection and the stability of the miRNA in milk. |
| Databáze: | OpenAIRE |
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