The Capsicum baccatum-Specific Truncated NLR Protein CbCN Enhances the Innate Immunity against Colletotrichum acutatum
| Autor: | Sang Ryeol Park, Jae Bok Yoon, Jae Wahng Do, Jung-Heon Han, Jun Oh, Seungmin Son, Kyong Sil Lee, Inchan Choi, Soohong Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Colletotrichum acutatum QH301-705.5 Nicotiana benthamiana NLR Proteins Genetically modified crops Plant disease resistance 01 natural sciences Catalysis Inorganic Chemistry 03 medical and health sciences Colletotrichum Gene silencing Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Gene Capsicum baccatum Spectroscopy Disease Resistance Plant Diseases Genetics biology Communication Organic Chemistry fungi food and beverages nucleotide-binding and leucine-rich repeat General Medicine anthracnose resistance NPR1 biology.organism_classification Computer Science Applications Chemistry 030104 developmental biology Host-Pathogen Interactions Disease Susceptibility Capsicum 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 7672, p 7672 (2021) |
| ISSN: | 1422-0067 |
| Popis: | Chili pepper (Capsicumannuum) is an important fruit and spice used globally, but its yield is seriously threatened by anthracnose. Capsicum baccatum is particularly valuable as it carries advantageous disease resistance genes. However, most of the genes remain to be identified. In this study, we identified the C. baccatum-specific gene CbCN, which encodes a truncated nucleotide-binding and leucine-rich repeat protein in the anthracnose resistant chili pepper variety PBC80. The transcription of CbCN was greater in PBC80 than it was in the susceptible variety An-S after Colletotrichum acutatum inoculation. In order to investigate the biological function of CbCN, we generated transgenic tobacco lines constitutively expressing CbCN. Notably, CbCN-overexpressing transgenic plants exhibited enhanced resistance to C. acutatum compared to wild-type plants. Moreover, the expression of pathogenesis-related (PR) genes was remarkably increased in a CbCN-overexpressing tobacco plants. In order to confirm these results in chili pepper, we silenced the CbCN gene using the virus-induced gene silencing system. The anthracnose resistance and expressions of PR1, PR2, and NPR1 were significantly reduced in CbCN-silenced chili peppers after C. acutatum inoculations. These results indicate that CbCN enhances the innate immunity against anthracnose caused by C. acutatum by regulating defense response genes. |
| Databáze: | OpenAIRE |
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