New strip immunoblot for the confirmation of HTLV-I/II infection

Autor: Robert K. Dinello, S. Pichuantes, H. W. Reesink, P. N. Lelie, H. Vrielink, Hans L. Zaaijer, H. T. M. Cuypers, D. Larson, Kuldip Sra, Stella Quan, Alan Polito, Maria Rios, C. L. Van Der Poel
Přispěvatelé: Other departments
Jazyk: angličtina
Rok vydání: 1996
Předmět:
Zdroj: Vox sanguinis, 70(2), 114-116. Wiley-Blackwell
ISSN: 0042-9007
Popis: In The Netherlands, anti-human T-cell lymphotropic virus type I (HTLV-I) blood donor screening became mandatory in January 1993. Donations reactive in the enzymelinked immunosorbent assay (ELISA) screening test are confirmed with Western blot analysis (WB). Only WB-positive or indeterminate blood donors are notified and retested by polymerase chain reaction (PCR) [1]. In accumulated data of the Dutch Blood Banks, only 2% of donors repeatedly positive in the anti-HTLV-I/II ELISA were WB-positive and all of these were also PCR-positive. However, 75% of the ELISA-positive blood donors have indeterminate WB results. In these cases the ELISA reactivities are nonspecific since all WB-indeterminate donors are negative in PCR [2]. Current WB confirmation thus leads to the notification and often to the deferral of many WB-indeterminate blood donors as well as to high costs for PCR testing, illustrating the need for a more specific serological confirmatory assay. The aim of our study was to evaluate a newly developed anti-HTLV-I/II Recombinant Immunoblot Assay (RIBA) to confirm samples reactive to screening tests with a special emphasis on the ability of this test to resolve WB-indeterminate results in blood donors, without compromising the sensitivity.
Databáze: OpenAIRE
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