Transformation vector based on promoter and intron sequences of a replacement histone H3 gene. A tool for high, constitutive gene expression in plants
| Autor: | Dénes Dudits, János Györgyey, Attila Fehér, Jakob H. Waterborg, Antal Mai, Tamas Kapros, Zsolt Kelemen |
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| Rok vydání: | 2002 |
| Předmět: |
Genetic Vectors
Polymerase Chain Reaction Histones Viral Proteins Histone H3 Transformation Genetic Caulimovirus Genes Reporter Tobacco Gene expression Genetics Promoter Regions Genetic Gene DNA Primers Glucuronidase Reporter gene Expression vector Base Sequence biology fungi Intron food and beverages Promoter Plants Genetically Modified biology.organism_classification Introns Animal Science and Zoology Cauliflower mosaic virus Agronomy and Crop Science Medicago sativa Biotechnology |
| Zdroj: | Transgenic Research. 11:69-72 |
| ISSN: | 1573-9368 0962-8819 |
| Popis: | This study explored the possibility of using non-viral, plant-based gene sequences to create strong and constitutive expression vectors. Replacement histone H3 genes are highly and constitutively expressed in all plants. Sequences of the cloned alfalfa histone H3.2 gene MsH3gl were tested. Constructs of the beta-glucuronidase (GUS) reporter gene were produced with H3.2 gene promoter and intron sequences. Their efficiency was compared with that of the commonly used strong 35S cauliflower mosaic virus promoter in transgenic tobacco plants. Combination of the H3.2 promoter and intron produced significantly higher GUS expression than the strong viral 35S promoter. Histochemical GUS analysis revealed a constitutive pattern of expression. Thus, alfalfa replacement H3 gene sequences can be used instead of viral promoters to drive heterologous gene expression in plants, avoiding perceived risks of viral sequences. |
| Databáze: | OpenAIRE |
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