Free fatty acids alter the electrophoretic mobility of serum lipoproteins
| Autor: | Yoshihei Hirasawa, Fumio Ogata |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Electrophoresis
Agar Gel Intermediate-density lipoprotein Chromatography Chemistry Biophysics Cell Biology Fatty Acids Nonesterified Biochemistry Lipoproteins LDL body regions Electrophoresis Negative charge Humans lipids (amino acids peptides and proteins) Lipoproteins HDL Ultracentrifugation Molecular Biology hormones hormone substitutes and hormone antagonists Lipoprotein Lipoprotein cholesterol |
| Zdroj: | Biochemical and Biophysical Research Communications. 107:417-421 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(82)91507-8 |
| Popis: | When normal human serum was analysed after addition of free fatty acids (FFA: oleic acids), the lipoproteins showed abnormal mobility on agarose-gel electrophoresis. β- and α-lipoproteins from the serum with a FFA concentration of 2.04 μEq/ml or more migrated with a mobility showing a more negative charge than native ones. Although the ultracentrifugal profile of the lipoprotein cholesterol was not altered with the excess-FFA, FFA were detected in the lipoprotein fractions as well as in free proteins. The lipoproteins isolated from the excess-FFA aerum also showed abnormal mobility on agarosegel electrophoresis. These results suggest that, in the serum containing excess-FFA, FFA attach themselves to the lipoproteins and alter their charge. |
| Databáze: | OpenAIRE |
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