Integrated analysis of recombinant BPV-1 L1 protein for the production of a bovine papillomavirus VLP vaccine
| Autor: | Alexandre Pereira, J. Mazzuchelli-de-Souza, Rodrigo Pinheiro Araldi, Letícia Maria Zanphorlin, Diego Grando Módolo, Marcelo Menossi, Willy Beçak, Daniel C. Pimenta, R.C. Stocco, R.F. Carvalho |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Protein Folding Protein Conformation viruses Cattle Diseases Antibodies Viral law.invention 03 medical and health sciences Papillomavirus Vaccines 0302 clinical medicine law Animals Vaccines Virus-Like Particle Bovine papillomavirus 1 Bovine papillomavirus Vaccines Synthetic General Veterinary General Immunology and Microbiology biology Protein Stability Bovine Papillomavirus-1 Immunogenicity Papillomavirus Infections Capsomere Public Health Environmental and Occupational Health biochemical phenomena metabolism and nutrition biology.organism_classification Virology Recombinant Proteins Vaccination 030104 developmental biology Infectious Diseases Capsid 030220 oncology & carcinogenesis Recombinant DNA Molecular Medicine Capsid Proteins Cattle Protein Multimerization |
| Zdroj: | Vaccine. 35:1590-1593 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2017.02.007 |
| Popis: | Bovine papillomatosis is an infectious disease that is caused by bovine papillomavirus (BPV), which results in important economic losses. However, no BPV vaccines or effective treatment methods are commercially available to date. Moreover, the absence of papillomavirus replication in vitro makes the use of recombinant protein a promising candidate for vaccine formulations. Hence, we developed an integrated study on the L1 capsid protein of BPV-1, obtained from a bacterial expression system, regarding its purification, biosafety, thermostability and immunogenicity. The results indicated an absence of genotoxicity of the purified recombinant L1 protein, β-sheet prevalence of secondary structure folding, protein stability under high temperatures as well as the presence of capsomeres and VLPs. In addition, preliminary experimental vaccination of calves showed the production of specific antibodies against BPV-1 L1. |
| Databáze: | OpenAIRE |
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