Direct activation of RIP3/MLKL-dependent necrosis by herpes simplex virus 1 (HSV-1) protein ICP6 triggers host antiviral defense
| Autor: | Zili Zhang, She Chen, Xiaoliang Yu, Lin Li, Xing Wang, Shan Liu, Lin Ge, Qin Chen, Lu Yu, Cuilin Shi, Sudan He, Lei Xu, Zhongxu Yang, Biqi Zhou, Yun Li, Wenhui He, Zhilin Hu, Xuejun Jiang, Jun Li |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Programmed cell death Necrosis Herpesvirus 1 Human Biology medicine.disease_cause Virus Replication Receptors Tumor Necrosis Factor Mice Viral Proteins medicine Animals Humans Receptor Glycoproteins Multidisciplinary Activator (genetics) NF-kappa B RNA-Binding Proteins Herpes Simplex Biological Sciences Virus Internalization Virology Cell biology Deubiquitinating Enzyme CYLD Protein Structure Tertiary Toll-Like Receptor 3 Enzyme Activation Cysteine Endopeptidases Herpes simplex virus Receptor-Interacting Protein Serine-Threonine Kinases TLR3 Host-Pathogen Interactions Tumor necrosis factor alpha Ectopic expression medicine.symptom Protein Kinases Protein Binding |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 111(43) |
| ISSN: | 1091-6490 |
| Popis: | The receptor-interacting kinase-3 (RIP3) and its downstream substrate mixed lineage kinase domain-like protein (MLKL) have emerged as the key cellular components in programmed necrotic cell death. Receptors for the cytokines of tumor necrosis factor (TNF) family and Toll-like receptors (TLR) 3 and 4 are able to activate RIP3 through receptor-interacting kinase-1 and Toll/IL-1 receptor domain-containing adapter inducing IFN-β, respectively. This form of cell death has been implicated in the host-defense system. However, the molecular mechanisms that drive the activation of RIP3 by a variety of pathogens, other than the above-mentioned receptors, are largely unknown. Here, we report that human herpes simplex virus 1 (HSV-1) infection triggers RIP3-dependent necrosis. This process requires MLKL but is independent of TNF receptor, TLR3, cylindromatosis, and host RIP homotypic interaction motif-containing protein DNA-dependent activator of IFN regulatory factor. After HSV-1 infection, the viral ribonucleotide reductase large subunit (ICP6) interacts with RIP3. The formation of the ICP6-RIP3 complex requires the RHIM domains of both proteins. An HSV-1 ICP6 deletion mutant failed to cause effective necrosis of HSV-1-infected cells. Furthermore, ectopic expression of ICP6, but not RHIM mutant ICP6, directly activated RIP3/MLKL-mediated necrosis. Mice lacking RIP3 exhibited severely impaired control of HSV-1 replication and pathogenesis. Therefore, this study reveals a previously uncharacterized host antipathogen mechanism. |
| Databáze: | OpenAIRE |
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