Polyanhydride Nanovaccines Induce Germinal Center B Cell Formation and Sustained Serum Antibody Responses
| Autor: | Neil S. Greenspan, Thomas J. Waldschmidt, Balaji Narasimhan, Michael W. Cho, Jihua Hao, Lorraine T. Tygrett, Habtom H. Habte, Julia E. Vela Ramirez |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antigen presentation Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Antibodies Article Mice 03 medical and health sciences Immune system Antigen Polyanhydrides medicine Animals Nanotechnology General Materials Science B cell B-Lymphocytes Mice Inbred BALB C biology Chemistry Germinal center T-Lymphocytes Helper-Inducer T helper cell Germinal Center 030104 developmental biology medicine.anatomical_structure Immunoglobulin class switching Vaccines Subunit Immunology biology.protein Nanoparticles Female Antibody |
| Zdroj: | Journal of Biomedical Nanotechnology. 12:1303-1311 |
| ISSN: | 1550-7033 |
| Popis: | Biodegradable polymeric nanoparticle-based subunit vaccines have shown promising characteristics by enhancing antigen presentation and inducing protective immune responses when compared with soluble protein. Specifically, polyanhydride nanoparticle-based vaccines (i.e., nanovaccines) have been shown to successfully encapsulate and release antigens, activate B and T cells, and induce both antibody- and cell-mediated immunity towards a variety of immunogens. One of the characteristics of strong thymus-dependent antibody responses is the formation of germinal centers (GC) and the generation of GC B cells, which is part of the T helper cell driven cellular response. In order to further understand the role of nanovaccines in the induction of antigen-specific immune responses, their ability to induce germinal center B cell formation and isotype switching and the effects thereof on serum antibody responses were investigated in these studies. Polyanhydride nanovaccines based on 1,6-bis(p-carboxyphenoxy)hexane and 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane were used to subcutaneously administer a viral antigen. GC B cell formation and serum antibody responses induced by the nanovaccines were compared to that induced by alum-based vaccine formulations. It was demonstrated that a single dose of polyanhydride nanovaccines resulted in the formation of robust GCs and serum antibody in comparison to that induced by the alum-based formulation. This was attributed to the sustained release of antigen provided by the nanovaccines. When administered in a multiple dose regimen, the highest post-immunization titer and GC B cell number was enhanced, and the immune response induced by the nanovaccines was further sustained. These studies provide foundational information on the mechanism of action of polyanhydride nanovaccines. |
| Databáze: | OpenAIRE |
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