CYTOTOXICITY AND MUTAGENICITY OF OPHTHALMIC SOLUTION PRESERVATIVES AND UVA RADIATION IN L5178Y CELLS
| Autor: | Victoria M. Hitchins, Thomas J. Withrow, Natalie T. Brown, Amparo G. Strickland |
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| Rok vydání: | 1989 |
| Předmět: |
Preservative
Cell Survival Ultraviolet Rays Ethylenediaminetetraacetic acid Biochemistry Benzalkonium chloride chemistry.chemical_compound Pharmaceutic Aids Tumor Cells Cultured medicine Animals Leukemia L5178 Physical and Theoretical Chemistry Cytotoxicity Spectrum Analysis Thimerosal Chlorhexidine Preservatives Pharmaceutical General Medicine Molecular biology chemistry Cell culture Thymidine kinase Mutation Immunology Ophthalmic Solutions medicine.drug |
| Zdroj: | Photochemistry and Photobiology. 50:385-389 |
| ISSN: | 1751-1097 0031-8655 |
| DOI: | 10.1111/j.1751-1097.1989.tb04174.x |
| Popis: | Four chemical preservatives commonly used in ophthalmic solutions were tested for their toxic and mutagenic potential in mouse lymphoma cells with and without exposure of the cells to ultraviolet A (UVA) radiation. The preservatives tested were benzalkonium chloride (BAK), chlorhexidine, thimerosal and ethylenediaminetetraacetic acid (EDTA). Cell survival and mutagenesis were measured using the L5178Y mouse lymphoma (TK +/-) system. Cells were exposed to varying amounts of preservatives for 1 h at 37 degrees C, and then aliquots were irradiated with UVA radiation (during the exposure to preservative). Cells were then assayed for survival, and for mutagenesis at the thymidine kinase (TK) locus. In concentrations commonly found in ophthalmic solutions, BAK, chlorhexidine, and thimerosal were toxic to cells, and thimerosal was slightly mutagenic. When cells were exposed to preservative and UVA radiation, chlorhexidine was mutagenic and the mutagenic activity of thimerosal was enhanced. |
| Databáze: | OpenAIRE |
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