Melatonin-pretreated adipose-derived mesenchymal stem cells efficeintly improved learning, memory, and cognition in an animal model of Alzheimer's disease
| Autor: | Ebrahim Nasiri, Nasrin Hashemi-Firouzi, Akram Alizadeh, Zoleikha Golipoor, Amaneh Mohammadi Roushandeh, Rouhollah Gazor |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_treatment Morris water navigation task Adipose tissue Pharmacology Mesenchymal Stem Cell Transplantation Biochemistry Antioxidants Cell therapy Melatonin 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Cognition Alzheimer Disease Memory medicine Avoidance Learning Animals Learning Rats Wistar Amyloid beta-Peptides business.industry Mesenchymal stem cell Mesenchymal Stem Cells Stem-cell therapy Rats Transplantation Disease Models Animal 030104 developmental biology Immunohistochemistry Neurology (clinical) business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Metabolic brain disease. 34(4) |
| ISSN: | 1573-7365 |
| Popis: | Currently, mesenchymal stem cells (MSCs) based therapy has extensive attraction for Alzheimer’s disease (AD). However, low survival rate of MSCs after transplantation is a huge challenging. The current study aimed to improve adipose-derived MSCs (AD-MSCs)-based therapy by their pre-treatment with melatonin (MT) ‘a well-known antioxidant’ in an animal model of AD. In this study, after isolating rat AD-MSCs from the epididymal white adipose tissues, the cells were pretreated with 5μM of MT for 24 hours. Forty male Wistar rats were randomly allocated to control, sham, amyloid-beta (Aβ) peptide, AD-MSCs and MT-pretreated ADMSCs groups. The novel object recognition, passive avoidance test, Morris water maze and open field test were performed two months following the cell transplantation. The rats were sacrificed 69 days following cell therapy. The brain tissues were removed for histopathological analysis and also immunohistochemistry was performed for two Aβ1-42 and Iba1 proteins. It has been revealed that both AD-MSCs and MT-AD-MSCs migrated to brain tissues after intravenous transplantation. However, MT-ADMSCs significantly improved learning, memory and cognition compared with AD-MSCs (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink