Increased expression of HDJ-2 (hsp40) in carotid artery atherosclerosis: A novel heat shock protein associated with luminal stenosis and plaque ulceration
| Autor: | Andrés Jaramillo, William F. Oppat, Gregorio A. Sicard, T. Mohanakumar, Suzanne Dintzis, Robert W. Thompson, Thelinh Q. Nguyen, Brent T. Allen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Carotid Artery Diseases
Pathology medicine.medical_specialty Arteriosclerosis medicine.medical_treatment Carotid endarterectomy Pathogenesis Lesion Central nervous system disease medicine Humans Carotid Stenosis HSP70 Heat-Shock Proteins RNA Messenger Heat-Shock Proteins Vascular disease business.industry Reverse Transcriptase Polymerase Chain Reaction Chaperonin 60 HSP40 Heat-Shock Proteins medicine.disease Immunohistochemistry Pathophysiology Hsp70 Stenosis Blotting Southern Carotid Arteries Surgery medicine.symptom business Cardiology and Cardiovascular Medicine |
| Zdroj: | Journal of Vascular Surgery. 33(5):1065-1071 |
| ISSN: | 0741-5214 |
| DOI: | 10.1067/mva.2001.113298 |
| Popis: | Purpose: Evidence suggests that both humoral and cellular autoimmune processes directed toward heat shock proteins (hsp) contribute to the pathogenesis of atherosclerosis. We characterized a human hsp distinct from those previously characterized in atherosclerotic lesions, termed HDJ-2. Methods: To determine the role of HDJ-2 in atherosclerosis, we compared the level of HDJ-2 mRNA expression with the level of hsp60 and hsp70 mRNA expression in 26 carotid endarterectomy specimens and 17 normal arteries. The level of expression of HDJ-2 mRNA was also correlated to the presence of plaque ulceration and the degree of luminal stenosis associated with the lesion. Results: The expression of HDJ-2 and hsp70 was significantly higher in carotid artery plaques as compared with normal arteries: HDJ-2, 6.7 ± 1.6 vs 0.1 ± 0.04, (P =.001); hsp70, 9.5 ± 0.9 vs 3.7 ± 0.8, (P =.002). There was no significant difference in hsp60 expression between carotid artery plaques and normal arteries (21.0 ± 0.9 vs 20.6 ± 0.8, P =.65). Increased HDJ-2 expression in carotid artery plaques was independent of hsp70 (Pearson correlation, r = 0.11; Bartlett χ2 analysis, P =.71). Within the ulcerated plaque group, there was a correlation between degree of stenosis and high HDJ-2 mRNA expression (r = 0.896, P =.016). However, there was no correlation between degree of stenosis and high HDJ-2 mRNA expression within the nonulcerated plaque group (r = 0.530, P =.076) or within the entire group of patients (r = 0.0085, P =.97). Conclusion: These results demonstrate that expression of HDJ-2 is significantly increased in atherosclerotic carotid artery plaques as compared with hsp60 and hsp70 and correlates with luminal stenosis in ulcerated atherosclerotic carotid artery plaques. (J Vasc Surg 2001;33:1065-71.) |
| Databáze: | OpenAIRE |
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