IL-4 directs both CD4 and CD8 T cells to produce Th2 cytokines in vitro, but only CD4 T cells produce these cytokines in response to alum-precipitated protein in vivo
Autor: | Roger Bird, Adam F. Cunningham, Ian C. M. MacLennan, Karine Serre, Peter J. L. Lane, Elodie Mohr, Fabrina Gaspal |
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Rok vydání: | 2010 |
Předmět: |
CD4-Positive T-Lymphocytes
Ovalbumin T cell Immunology Mice Transgenic Cell Separation CD8-Positive T-Lymphocytes Biology Lymphocyte Activation Article Mice Interleukin 21 Th2 Cells medicine Animals Immunologic Factors Cytotoxic T cell IL-2 receptor Antigen-presenting cell Molecular Biology Interleukin 3 Reverse Transcriptase Polymerase Chain Reaction ZAP70 Flow Cytometry Natural killer T cell Molecular biology Peptide Fragments Mice Inbred C57BL medicine.anatomical_structure Alum Compounds Cytokines Interleukin-4 |
Zdroj: | Molecular Immunology. 47:1914-1922 |
ISSN: | 0161-5890 |
Popis: | While IL-4 directs CD4 T cells to produce Th2 cytokines (including IL-4, IL-13, IL-5) in vitro it has been shown that production of these cytokines can be induced in vivo in the absence of IL-4/IL-13/STAT-6 signaling. The present report shows that CD8 as well as CD4 T cells activated through their TCR, in vitro upregulate the Th2-features - IL-4, IL-13, IL-5, and GATA-3. However, in vivo while alum-precipitated antigen strongly and selectively induces these Th2-features in CD4 T cells, CD8 T cells mount a markedly different response to this antigen. This CD8 response is associated with strong proliferation and production of IFN-gamma, but no Th2-features are induced. Alum-protein formulations are widely used in human vaccines and typically induce strong antibody responses characterized by the differentiation of IL-4-producing CD4 T cells and immunoglobulin class switching to IgG1. Nevertheless, the mechanism responsible for CD4 Th2 and follicular helper T cell commitment triggered by these alum-protein vaccines is still poorly understood. Analysis of the in vivo response to alum-precipitated protein shows that while subsets of CD4 T cells strongly upregulate Th2 and follicular helper T cell features including the surface markers OX40, CXCR5, PD-1, IL-17RB and the transcription factor c-Maf, CD8 T cells do not. These discrete differences between responding CD4 and CD8 T cells provide further insight into the differences between Th2 polarization of CD4 T cells directed by IL-4 in vitro and the induction of IL-4 production by CD4 T cells in vivo in response to alum-precipitated protein. |
Databáze: | OpenAIRE |
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