A rat model of serous borderline ovarian tumors induced by 7,12-dimethylbenz[a]anthracene

Autor: Jin-Wei Qiang, Shu-Hui Zhao, Song-Qi Cai, Li Wang, Yong-Ai Li, Ying Li, Xin Li, Jian Zhu
Rok vydání: 2019
Předmět:
0301 basic medicine
dimethylbenz[a]anthracene
Time Factors
endocrine system diseases
Original
9
10-Dimethyl-1
2-benzanthracene
DMBA
General Biochemistry
Genetics and Molecular Biology
Rats
Sprague-Dawley
Pathogenesis
Random Allocation
Sprague-Dawley rat
03 medical and health sciences
Ovarian tumor
chemistry.chemical_compound
0302 clinical medicine
Cyclin D1
serous borderline tumor
Animals
Medicine
Ovarian Neoplasms
Dose-Response Relationship
Drug
General Veterinary
medicine.diagnostic_test
business.industry
animal model
7
12-Dimethylbenz[a]anthracene
Magnetic resonance imaging
General Medicine
Rats
Disease Models
Animal
Serous fluid
030104 developmental biology
chemistry
ovarian tumor
Carcinogens
Cancer research
Biomarker (medicine)
Female
Animal Science and Zoology
business
030217 neurology & neurosurgery
Zdroj: Experimental Animals
ISSN: 1881-7122
1341-1357
Popis: Serous borderline ovarian tumors (SBOTs) behave between benign cystadenomas and carcinomas, and the effective detection and clinical management of SBOTs remain clinical challenges. Because it is difficult to isolate and enrich borderline tumor cells, a borderline animal model is in need. 7,12-dimethylbenz[a]anthracene (DMBA) is capable of inducing the initiation, promotion, and progression of serous ovarian tumors. This study aims to investigate the proper dosage and induction time of DMBA for rat models of SBOTs, and explore their morphological features demonstrated by magnetic resonance (MR) imaging and molecular genetic characteristics. Rats were randomly divided into six groups (1 mg/70 D, 2 mg/70 D, 3 mg/70 D, 2 mg/50 D, 2 mg/90 D, and 2 mg/110 D). The 3 mg/70 D group induced the most SBOTs (50.0%, 12/24). The micropapillary projections were shown on MR imaging, which was the characteristic of SBOTs. The Cyclin D1 characterizing an early pathogenetic event strongly expressed in induced serous benign tumors (SBTs). The immunoreactivity staining scores of P53 expression significantly increased from SBTs, SBOTs to serous ovarian carcinomas (SCAs), which elucidate that P53 might be a promising biomarker to grade serous ovarian tumors. Based on morphological and molecular genetic similarities, this rodent SBOT model was suitable for investigating the pathogenesis of serous ovarian tumors and developing an early detection strategy.
Databáze: OpenAIRE
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