A rat model of serous borderline ovarian tumors induced by 7,12-dimethylbenz[a]anthracene
Autor: | Jin-Wei Qiang, Shu-Hui Zhao, Song-Qi Cai, Li Wang, Yong-Ai Li, Ying Li, Xin Li, Jian Zhu |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
dimethylbenz[a]anthracene Time Factors endocrine system diseases Original 9 10-Dimethyl-1 2-benzanthracene DMBA General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley Pathogenesis Random Allocation Sprague-Dawley rat 03 medical and health sciences Ovarian tumor chemistry.chemical_compound 0302 clinical medicine Cyclin D1 serous borderline tumor Animals Medicine Ovarian Neoplasms Dose-Response Relationship Drug General Veterinary medicine.diagnostic_test business.industry animal model 7 12-Dimethylbenz[a]anthracene Magnetic resonance imaging General Medicine Rats Disease Models Animal Serous fluid 030104 developmental biology chemistry ovarian tumor Carcinogens Cancer research Biomarker (medicine) Female Animal Science and Zoology business 030217 neurology & neurosurgery |
Zdroj: | Experimental Animals |
ISSN: | 1881-7122 1341-1357 |
Popis: | Serous borderline ovarian tumors (SBOTs) behave between benign cystadenomas and carcinomas, and the effective detection and clinical management of SBOTs remain clinical challenges. Because it is difficult to isolate and enrich borderline tumor cells, a borderline animal model is in need. 7,12-dimethylbenz[a]anthracene (DMBA) is capable of inducing the initiation, promotion, and progression of serous ovarian tumors. This study aims to investigate the proper dosage and induction time of DMBA for rat models of SBOTs, and explore their morphological features demonstrated by magnetic resonance (MR) imaging and molecular genetic characteristics. Rats were randomly divided into six groups (1 mg/70 D, 2 mg/70 D, 3 mg/70 D, 2 mg/50 D, 2 mg/90 D, and 2 mg/110 D). The 3 mg/70 D group induced the most SBOTs (50.0%, 12/24). The micropapillary projections were shown on MR imaging, which was the characteristic of SBOTs. The Cyclin D1 characterizing an early pathogenetic event strongly expressed in induced serous benign tumors (SBTs). The immunoreactivity staining scores of P53 expression significantly increased from SBTs, SBOTs to serous ovarian carcinomas (SCAs), which elucidate that P53 might be a promising biomarker to grade serous ovarian tumors. Based on morphological and molecular genetic similarities, this rodent SBOT model was suitable for investigating the pathogenesis of serous ovarian tumors and developing an early detection strategy. |
Databáze: | OpenAIRE |
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