Visualization of Endogenous Type I TGF-β Receptor Baboon in the Drosophila Brain
| Autor: | Po-Lin Chen, Yen-Wei Lai, Jian-Chiuan Li, Sao-Yu Chu, Chun-Hong Chen, Hung-Hsiang Yu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Untranslated region Neurite Activin Receptors lcsh:Medicine Hemagglutinin Glycoproteins Influenza Virus Biology Article Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Transforming Growth Factor beta Neurites Animals Drosophila Proteins Gene Knock-In Techniques Receptor lcsh:Science Mushroom Bodies Regulation of gene expression Multidisciplinary lcsh:R Brain Gene Expression Regulation Developmental Axons Activins Cell biology MicroRNAs 030104 developmental biology Mushroom bodies Neuronal development Drosophila Biological metamorphosis lcsh:Q Signal transduction Carrier Proteins 030217 neurology & neurosurgery Signal Transduction Transforming growth factor |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-7 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The transforming growth factor β (TGF-β) signaling pathway is evolutionarily conserved and widely used in the animal kingdom to regulate diverse developmental processes. Prior studies have shown that Baboon (Babo), a Drosophila type I TGF-β receptor, plays essential roles in brain development and neural circuit formation. However, the expression pattern for Babo in the developing brain has not been previously reported. We generated a knock-in fly with a human influenza hemagglutinin (HA) tag at the C-terminus of Babo and assessed its localization. Babo::HA was primarily expressed in brain structures enriched with neurites, including the mushroom body lobe and neuropils of the optic lobe, where Babo has been shown to instruct neuronal morphogenesis. Since the babo 3' untranslated region contains a predicted microRNA-34 (miR-34) target sequence, we further tested whether Babo::HA expression was affected by modulating the level of miR-34. We found that Babo was upregulated by mir-34 deletion and downregulated by miR-34 overexpression, confirming that it is indeed a miR-34 target gene. Taken together, our results demonstrate that the baboHA fly permits accurate visualization of endogenous Babo expression during brain development and the construction of functional neural circuits. |
| Databáze: | OpenAIRE |
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