Interaction of N-acetyl-phenylalanyl-tRNAPhe with 70S ribosomes of Escherichia coli
| Autor: | S.V. Kirillov, Vladimir B. Odinzov |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Poly U
Stereochemistry Phenylalanine Enthalpy Peptide Chain Elongation Translational chemistry.chemical_element Biology RNA Transfer Amino Acyl medicine.disease_cause Ribosome Genetics medicine Escherichia coli P-site RNA Messenger Binding site Binding Sites Magnesium Acetylation A-site Biochemistry chemistry Thermodynamics Ribosomes |
| Zdroj: | Nucleic acids research. 5(10) |
| ISSN: | 0305-1048 |
| Popis: | The interaction of N--Acetyl--Phe--tRNA Phe with 70 S ribosomes is a reversible process in the absence as well as in the presence of messenger. The equilibrium binding constants of these interactions were measured at different magnesium concentrations and temperatures and thermodynamical quantities computed. The enthalpy of the formation of complexes with the P site of ribosomes is larger by 6,000 cal/mol in the presence of poly (U) than in the presence of poly (C) or in total absence of messenger. Free energy differences are rather small, the association constants differ less than one order of magnitude. The association constant of N--Acetyl--Phe--tRNA Phe with the A site of ribosomes is 30--50 times lower than with the P site even in the presence of poly (U). |
| Databáze: | OpenAIRE |
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