Marked bias towards spontaneous synaptic inhibition distinguishes non-adapting from adapting layer 5 pyramidal neurons in the barrel cortex
| Autor: | Ion R. Popescu, Rebecca L. Voglewede, Ricardo Mostany, Kathy Q. Le, Rocío Palenzuela |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Postsynaptic Current Presynaptic inhibition lcsh:Medicine Machine learning computer.software_genre Inhibitory postsynaptic potential 03 medical and health sciences 0302 clinical medicine lcsh:Science Multidisciplinary Chemistry business.industry lcsh:R food and beverages Spike frequency adaptation Barrel cortex Electrophysiology 030104 developmental biology Slow afterhyperpolarization nervous system Excitatory postsynaptic potential lcsh:Q Artificial intelligence business Neuroscience computer 030217 neurology & neurosurgery |
| Zdroj: | DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria instname DDFV: Repositorio Institucional de la Universidad Francisco de Vitoria Universidad Francisco de Vitoria Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
| Popis: | Pyramidal neuron subtypes differ in intrinsic electrophysiology properties and dendritic morphology. However, do different pyramidal neuron subtypes also receive synaptic inputs that are dissimilar in frequency and in excitation/inhibition balance? Unsupervised clustering of three intrinsic parameters that vary by cell subtype – the slow afterhyperpolarization, the sag, and the spike frequency adaptation – split layer 5 barrel cortex pyramidal neurons into two clusters: one of adapting cells and one of non-adapting cells, corresponding to previously described thin- and thick-tufted pyramidal neurons, respectively. Non-adapting neurons presented frequencies of spontaneous inhibitory postsynaptic currents (sIPSCs) and spontaneous excitatory postsynaptic currents (sEPSCs) three- and two-fold higher, respectively, than those of adapting neurons. The IPSC difference between pyramidal subtypes was activity independent. A subset of neurons were thy1-GFP positive, presented characteristics of non-adapting pyramidal neurons, and also had higher IPSC and EPSC frequencies than adapting neurons. The sEPSC/sIPSC frequency ratio was higher in adapting than in non-adapting cells, suggesting a higher excitatory drive in adapting neurons. Therefore, our study on spontaneous synaptic inputs suggests a different extent of synaptic information processing in adapting and non-adapting barrel cortex neurons, and that eventual deficits in inhibition may have differential effects on the excitation/inhibition balance in adapting and non-adapting neurons. pre-print 3365 KB |
| Databáze: | OpenAIRE |
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