Intranasal instillation of distilled water, hypertonic saline and sodium bicarbonate promotes redox imbalance and acute lung inflammation in adult mice
| Autor: | Guilherme de Paula Costa, Sophia Dias Pozzolini Fróes, Natália Alves de Matos, André Talvani, Ana Beatriz Farias de Souza, Frank Silva Bezerra, Nicole Elizabeth Philips, Sílvia Dantas Cangussú |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Physiology medicine.medical_treatment Inflammation Pharmacology medicine.disease_cause Interferon-gamma Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Saline Administration Intranasal Chemokine CCL2 Parenchymal Tissue Distillation Saline Solution Hypertonic Lung Sodium bicarbonate biology Tumor Necrosis Factor-alpha Chemistry General Neuroscience Water Pneumonia Hypertonic saline Mice Inbred C57BL Instillation Drug Sodium Bicarbonate medicine.anatomical_structure 030228 respiratory system Catalase Acute Disease biology.protein Nasal administration Saline Solution medicine.symptom Oxidation-Reduction 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Respiratory Physiology & Neurobiology. 266:27-32 |
| ISSN: | 1569-9048 |
| DOI: | 10.1016/j.resp.2019.04.013 |
| Popis: | Bronchial obstruction, caused by retained secretions, is often treated by the administration of mucoactive agents including distilled water, saline, hypertonic saline, and sodium bicarbonate. However, the inflammatory effect of these solutions on the lungs remains unclear. This study evaluated the instillation effects of different solutions on oxidative stress and lung inflammatory response in C57BL/6 mice. Fifty C57BL/6 mice were divided into 5 groups: control (CG); distilled water (DWG), hypertonic saline (HSG), saline (SG) and sodium bicarbonate (SBG). CG was exposed to ambient air while DWG, HSG, SG and SBG had 50 μl of respective solutions administered intranasally for 5 consecutive days. Twenty-four hours after the last intranasal instillation, all animals were euthanized for subsequent analysis. All solutions promoted increased recruitment of inflammatory cells to the lung compared to controls. Superoxide dismutase activity was lower in HSG compared to all other groups; catalase activity was reduced in SG, while it increased in SBG and DWG compared to CG. Finally, there was an increase in the inflammatory markers TNF-α, CCL2 and IFN-γ in DWG compared to CG, SG and HSG. In conclusions, the intranasal instillation of different solutions promotes redox imbalance and inflammation on lungs of adult mice. |
| Databáze: | OpenAIRE |
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