Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm
| Autor: | Jing Hua Hu, Xiao Bing He, Yuan Chang Yan |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
GABA Plasma Membrane Transport Proteins endocrine system Direct evidence Acrosome reaction Organic Anion Transporters Biology Testis medicine Animals RNA Messenger Molecular Biology gamma-Aminobutyric Acid reproductive and urinary physiology Messenger RNA Spermatid Reverse Transcriptase Polymerase Chain Reaction urogenital system Membrane Proteins Membrane Transport Proteins Cell Biology Immunohistochemistry Spermatozoa Molecular biology Sperm Rats Blot medicine.anatomical_structure GABAergic Carrier Proteins |
| Zdroj: | Cell Research. 10:51-58 |
| ISSN: | 1748-7838 1001-0602 |
| DOI: | 10.1038/sj.cr.7290035 |
| Popis: | Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence of 67kDa GAT1 protein and mRNA in rat testis by Western blotting and reverse transcription-polymerase chain reaction. Meanwhile, immunohistochemical and immunofluorescent analyses also identified GAT1 protein on the elongated spermatid and sperm. These results indicated that rat testis is a novel site of gat1 expression. Further studies should be taken to explore the role of GAT1 protein on sperm acrosome reaction. |
| Databáze: | OpenAIRE |
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