Inhibiting Gene Expression with Locked Nucleic Acids (LNAs) That Target Chromosomal DNA
| Autor: | David R. Corey, Rosalyn Ram, Brett P. Monia, Sylvie Gabillet, Khalil Arar, Randall L. Beane |
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| Rok vydání: | 2007 |
| Předmět: |
Molecular Sequence Data
Oligonucleotides Breast Neoplasms Biology Transfection Biochemistry Chromosomes Article Inhibitory Concentration 50 chemistry.chemical_compound Cell Line Tumor Nucleic Acids Complementary DNA Androgen Receptor Antagonists Humans Locked nucleic acid Promoter Regions Genetic Base Sequence Dose-Response Relationship Drug Molecular Structure Oligonucleotide Gene targeting Promoter DNA Molecular biology Gene Expression Regulation Neoplastic DNA binding site chemistry Receptors Androgen Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Gene Targeting Nucleic acid Female Receptors Progesterone |
| Zdroj: | Biochemistry. 46:7572-7580 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi700227g |
| Popis: | Oligonucleotides containing locked nucleic acid bases (LNAs) have increased affinity for complementary DNA sequences. We hypothesized that enhanced affinity might allow LNAs to recognize chromosomal DNA inside human cells and inhibit gene expression. To test this hypothesis, we synthesized antigene LNAs (agLNAs) complementary to sequences within the promoters of progesterone receptor (PR) and androgen receptor (AR). We observed inhibition of AR and PR expression by agLNAs but not by analogous oligomers containing 2'-methoxyethyl bases or noncomplementary LNAs. Inhibition was dose dependent and exhibited IC50 values of |
| Databáze: | OpenAIRE |
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