Coupling of retrograde flow to force production during malaria parasite migration
| Autor: | Katharina A. Quadt, Catherine A Moreau, Martin Streichfuss, Friedrich Frischknecht, Joachim P. Spatz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Plasmodium berghei Actin filament organization Protozoan Proteins General Physics and Astronomy Nanotechnology macromolecular substances Biology 03 medical and health sciences Mice 0302 clinical medicine Cell Movement Myosin Parasite hosting Animals General Materials Science Contact dynamics Actin General Engineering Forward locomotion Actins 3. Good health Biomechanical Phenomena Malaria Coupling (electronics) 030104 developmental biology Optical tweezers Sporozoites Biophysics 030217 neurology & neurosurgery |
| Zdroj: | ACS Nano |
| Popis: | Migration of malaria parasites is powered by a myosin motor that moves actin filaments, which in turn link to adhesive proteins spanning the plasma membrane. The retrograde flow of these adhesins appears to be coupled to forward locomotion. However, the contact dynamics between the parasite and the substrate as well as the generation of forces are complex and their relation to retrograde flow is unclear. Using optical tweezers we found retrograde flow rates up to 15 μm/s contrasting with parasite average speeds of 1–2 μm/s. We found that a surface protein, TLP, functions in reducing retrograde flow for the buildup of adhesive force and that actin dynamics appear optimized for the generation of force but not for maximizing the speed of retrograde flow. These data uncover that TLP acts by modulating actin dynamics or actin filament organization and couples retrograde flow to force production in malaria parasites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|