Real-time polymerase chain reaction detection of Lichtheimia species in bandages associated with cutaneous mucormycosis in burn patients

Autor: Fréalle, E., Rocchi, Steffi, Bacus, Morgane, Bachelet, H., Pasquesoone, Louise, Tavernier, B., Mathieu, D., Millon, Laurence, Bellanger, Anne-Pauline, Jeanne, Mathieu
Přispěvatelé: Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Département Anesthésie-Réanimation, Hôpital Roger Salengro, CHRU de Lille, Hôpital Roger Salengro, CHRU de Lille, Lille, France, CHU Lille, Institut de Pharmacie, Service de chirurgie plastique, esthétique et reconstructrice, Hôpital Roger Salengro [Lille], Institut Universitaire de Technologie - Aix-Marseille (IUT AMU), Aix Marseille Université (AMU), This study was funded by the University of Lille, the ‘Institut national de la santé et de la recherche médicale’ (Inserm), the ‘Centre National de la Recherche Scientifique’ (CNRS), the Pasteur Institute of Lille, and Lille University Hospital Center., The authors are grateful to M. Deckmyn for contribution to the collection of patients' epidemiological, clinical, mycological, and therapeutic data., Centre d’Infection et d’Immunité de Lille (CIIL) - U1019 - UMR 8204 ( CIIL ), Institut Pasteur de Lille, Réseau International des Instituts Pasteur ( RIIP ) -Réseau International des Instituts Pasteur ( RIIP ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IFR142-Université de Lille-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Aix-Marseille Université - Institut universitaire de technologie ( IUT AMU ), Aix Marseille Université ( AMU ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Microbiology (medical)
Mucorales
Adult
Male
medicine.medical_specialty
Lichtheimia corymbifera
030106 microbiology
Burn
Real-Time Polymerase Chain Reaction
[ SDV.EE.SANT ] Life Sciences [q-bio]/Ecology
environment/Health
Hospitals
University
Cutaneous mucormycosis
03 medical and health sciences
Bandage
Mucorales qPCR
0302 clinical medicine
Absidia
Medicine
Dermatomycoses
Humans
Mucormycosis
030212 general & internal medicine
Aged
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
biology
business.industry
General Medicine
Middle Aged
biology.organism_classification
medicine.disease
University hospital
Dermatology
Bandages
3. Good health
Lichtheimia species
Infectious Diseases
Real-time polymerase chain reaction
Molecular Diagnostic Techniques
Female
France
business
Burns
Zdroj: Journal of Hospital Infection
Journal of Hospital Infection, WB Saunders, 2018, 99 (1), pp.68-74. ⟨10.1016/j.jhin.2018.02.004⟩
Journal of Hospital Infection, WB Saunders, 2018, 99 (1), pp.68-74. 〈10.1016/j.jhin.2018.02.004〉
Journal of Hospital Infection, 2018, 99 (1), pp.68-74. ⟨10.1016/j.jhin.2018.02.004⟩
ISSN: 0195-6701
1532-2939
Popis: Summary Background Cutaneous mucormycoses, mainly due to Lichtheimia (Absidia), have occurred on several occasions in the Burn Unit of the University Hospital of Lille, France. Aim To investigate the potential vector role of non-sterile bandages used to hold in place sterile gauze used for wound dressing. Methods Mycological analysis by conventional culture, Mucorales real-time polymerase chain reaction (qPCR), and Lichtheimia species-specific qPCR were performed on eight crepe and six elasticized bandages that were sampled on two independent occasions in March 2014 and July 2016. Characteristics of the seven Lichtheimia mucormycoses which occurred in burn patients between November 2013 and July 2016 were also collected to assess the epidemiological relationship between potentially contaminated bandages and clinical infections. Findings One Lichtheimia corymbifera strain was isolated from a crepe bandage by culture, and Lichtheimia spp. qPCR was positive in six out of eight crepe and four out of six elasticized bandages. Using species-specific qPCR, Lichtheimia ramosa, Lichtheimia ornata, and L. corymbifera were identified in six out of ten, five out of ten, and four out of ten bandages, respectively. In patients with mucormycosis, L. ramosa and L. ornata were present in five and two cases, respectively. Conclusion Our data support the utility of Mucorales qPCR for epidemiological investigations, the potential role of these bandages in cutaneous mucormycoses in burn patients in our centre, and, consequently, the need for sterile bandages for the dressing of extensive wounds.
Databáze: OpenAIRE
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