Rat cytomegalovirus open reading frame R44 is an early-late gene that encodes a nuclear protein
| Autor: | Suzanne J.F. Kaptein, Gert Grauls, Cathrien A. Bruggeman, Erik Beuken, Cornelis Vink |
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| Rok vydání: | 2001 |
| Předmět: |
Human cytomegalovirus
Messenger RNA Genes Viral Binding protein Congenital cytomegalovirus infection Cytomegalovirus Nuclear Proteins General Medicine Biology medicine.disease Virology Molecular biology Rats law.invention Molecular Weight Open Reading Frames Open reading frame law Recombinant DNA medicine Animals Nuclear protein Gene |
| Zdroj: | Archives of Virology. 146:2211-2218 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s007050170031 |
| Popis: | Rat cytomegalovirus (RCMV) open reading frame R44 is the homolog of human cytomegalovirus gene UL44, which encodes the DNA polymerase accessory protein. Here, we show that R44 is transcribed as a 3.6-kb mRNA within the early and late phases of infection in vitro. In order to find potential monoclonal antibodies (MoAbs) directed against the R44-encoded protein (pR44), a panel of anti-RCMV MoAbs was screened for binding to pR44 recombinant proteins. Thus, an anti-pR44 MoAb, termed RCMV8, was identified. By using this MoAb, pR44 could be detected as early as 8 h after RCMV infection in vitro. The pR44 protein was determined to have a molecular mass of approximately 55 kDa and was found to be localized to the nucleus of RCMV-infected cells. |
| Databáze: | OpenAIRE |
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