A Novel Formulation of Glucose-Sparing Peritoneal Dialysis Solutions with L-Carnitine Improves Biocompatibility on Human Mesothelial Cells

Autor: Giuseppe Procino, Arduino Arduini, Monica Carmosino, Giuseppe Castellano, Francesca Piccapane, Mario Bonomini, Maria Svelto, Andrea Gerbino
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
medicine.medical_treatment
030232 urology & nephrology
Ultrafiltration
Biocompatible Materials
Pharmacology
Epithelium
lcsh:Chemistry
0302 clinical medicine
Dialysis Solutions
L-carnitine
peritoneal dialysis solution
lcsh:QH301-705.5
Spectroscopy
Microscopy
Confocal
Chemistry
General Medicine
Computer Science Applications
xylitol
medicine.anatomical_structure
peritoneal dialysis
Cytokines
Peritoneum
medicine.drug
Biocompatibility
Cell Survival
Catalysis
Article
Peritoneal dialysis
Tight Junctions
Inorganic Chemistry
03 medical and health sciences
mesothelium
Carnitine
medicine
Humans
Viability assay
Physical and Theoretical Chemistry
Molecular Biology
Inflammation
Organic Chemistry
Mesothelium
Ultrafiltration (renal)
Bicarbonates
030104 developmental biology
Glucose
lcsh:Biology (General)
lcsh:QD1-999
Cell culture
Kidney Failure
Chronic
glucose-sparing
Mesothelial Cell
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 123, p 123 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 1
ISSN: 1661-6596
1422-0067
Popis: The main reason why peritoneal dialysis (PD) still has limited use in the management of patients with end-stage renal disease (ESRD) lies in the fact that the currently used glucose-based PD solutions are not completely biocompatible and determine, over time, the degeneration of the peritoneal membrane (PM) and consequent loss of ultrafiltration (UF). Here we evaluated the biocompatibility of a novel formulation of dialytic solutions, in which a substantial amount of glucose is replaced by two osmometabolic agents, xylitol and l-carnitine. The effect of this novel formulation on cell viability, the integrity of the mesothelial barrier and secretion of pro-inflammatory cytokines was evaluated on human mesothelial cells grown on cell culture inserts and exposed to the PD solution only at the apical side, mimicking the condition of a PD dwell. The results were compared to those obtained after exposure to a panel of dialytic solutions commonly used in clinical practice. We report here compelling evidence that this novel formulation shows better performance in terms of higher cell viability, better preservation of the integrity of the mesothelial layer and reduced release of pro-inflammatory cytokines. This new formulation could represent a step forward towards obtaining PD solutions with high biocompatibility.
Databáze: OpenAIRE
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