An ion beam–induced Arabidopsis mutant with marked chromosomal rearrangement
| Autor: | Sachihiro Matsunaga, Vo Thi Thuong Lan, Ayako N. Sakamoto, Atsushi Tanaka, Satoru Fujimoto |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chromosomal rearrangement Ultraviolet Rays Health Toxicology and Mutagenesis Mutant Arabidopsis Chromosomal rearrangement Biology DNA Ribosomal Polymerase Chain Reaction Chromosomes Plant 03 medical and health sciences 0302 clinical medicine FISH Chromosome Segregation Regular Paper Radiology Nuclear Medicine and imaging Ribosomal DNA Base Pairing NHEJ Crosses Genetic Genetics Chromosome Aberrations Ions Recombination Genetic Radiation Zygote Mutation breeding ion beams Chromosome biology.organism_classification 030104 developmental biology Fertility Chromosome 3 030220 oncology & carcinogenesis Mutation |
| Zdroj: | Journal of Radiation Research |
| ISSN: | 1349-9157 0449-3060 |
| Popis: | Ion beams have been used as an effective tool in mutation breeding for the creation of crops with novel characteristics. Recent analyses have revealed that ion beams induce large chromosomal alterations, in addition to small mutations comprising base changes or frameshifts. In an effort to understand the potential capability of ion beams, we analyzed an Arabidopsis mutant possessing an abnormal genetic trait. The Arabidopsis mutant uvh3-2 is hypersensitive to UVB radiation when photoreactivation is unavailable. uvh3-2 plants grow normally and produce seeds by self-pollination. SSLP and CAPS analyses of F2 plants showed abnormal recombination frequency on chromosomes 2 and 3. PCR-based analysis and sequencing revealed that one-third of chromosome 3 was translocated to chromosome 2 in uvh3-2. FISH analysis using a 180 bp centromeric repeat and 45S ribosomal DNA (rDNA) as probes showed that the 45S rDNA signal was positioned away from that of the 180 bp centromeric repeat in uvh3-2, suggesting the insertion of a large chromosome fragment into the chromosome with 45S rDNA clusters. F1 plants derived from a cross between uvh3-2 and wild-type showed reduced fertility. PCR-based analysis of F2 plants suggested that reproductive cells carrying normal chromosome 2 and uvh3-2–derived chromosome 3 are unable to survive and therefore produce zygote. These results showed that ion beams could induce marked genomic alterations, and could possibly lead to the generation of novel plant species and crop strains. |
| Databáze: | OpenAIRE |
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