Microsatellite instability in solitary and sporadic gastric cancer
| Autor: | Ulysses Ribeiro, Bruno Zilberstein, Angelita Habr-Gama, Joaquim Gama-Rodrigues, Adriana Vaz Safatle Ribeiro, José Eduardo Krieger, Conrado Alvarenga, Rodrigo Oliva Perez, Cláudio José Caldas Bresciani, Carlos Eduardo Jacob, Fabricio L'ofreddo D'Ottaviano |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Genetic Markers Male Pathology medicine.medical_specialty Adenocarcinoma medicine.disease_cause Disease-Free Survival Genomic Instability Metastasis Stomach Neoplasms Genetics medicine Humans Radical surgery Survival analysis Aged Neoplasm Staging Aged 80 and over business.industry Stomach Microsatellite instability Cancer General Medicine Middle Aged medicine.disease Survival Analysis digestive system diseases medicine.anatomical_structure Genetic marker Female Gastric cancer Carcinogenesis business Brazil Microsatellite Repeats |
| Zdroj: | Revista do Hospital das Clínicas v.59 n.5 2004 Revista do Hospital das Clínicas Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0041-8781 |
| DOI: | 10.1590/s0041-87812004000500010 |
| Popis: | Recently, the presence of microsatellite instability (MSI) has been reported in gastric cancer and associated with older age of presentation, distal tumor location, early disease staging, and better overall prognosis. Different characteristics in presentation and in tumor behavior may be explained by different genetic alterations during carcinogenesis of gastric cancer. Identification of specific genetic pathways in gastric cancer may have direct impact on prognosis and selection of treatment strategies. PATIENTS AND METHODS: All 24 patients were treated by radical surgery. Fragments of normal and tumor tissues were extracted from the specimen and stored at -80ºC before DNA purification and extraction. PCR amplification utilizing microsatellite markers was performed. Tumors presenting PCR products of abnormal sizes were considered positive for microsatellite instability (MSI+). RESULTS: Five patients (21%) had tumors that were MSI+ in at least 1 marker. In the group of patients with Lauren's intestinal-type gastric carcinoma, 3 had tumors that were MSI+ (23%), while in the group of diffuse-type gastric cancer, 2 patients had tumors that were MSI+ (19%). The mean age of presentation and the male:female ratio was similar in both groups. Tumors that were MSI+ were more frequently located in proximal portion of the stomach compared to microsatellite-stable (MSS) tumors (40% vs. 16%). Although there was a trend of patients with MSI+ tumors towards a proximal gastric tumor location, early staging, and negative lymph node metastasis, there was no statistical significance compared to those with MSS tumors (P >.1). Comparison of overall and disease-free survival between gastric tumors that were MSI+ and those that were MSS found no statistically significant differences (P >.1). CONCLUSIONS: Microsatellite instability is a frequent event in gastric carcinogenesis and shows a trend towards distinct clinical and pathological characteristics of gastric cancer. |
| Databáze: | OpenAIRE |
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