Developmentally regulated mouse gene NK10 encodes a zinc finger repressor protein with differential DNA-binding domains
| Autor: | Dagmar Barthels, Markus Plomann, Harold Cremer, Gaby Vopper, Uwe A.O. Heinlein, Rita Lange, Kenneth R. Johnson, Hans-Jürgen Thiesen, Annette Christoph, Lydia Lemaire |
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| Rok vydání: | 1995 |
| Předmět: |
Recombinant Fusion Proteins
Molecular Sequence Data Repressor Biology ZIC2 Mice Open Reading Frames Genetics Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Gene LIM domain Zinc finger Base Sequence Oligonucleotide Chromosome Mapping Gene Expression Regulation Developmental Zinc Fingers Cell Biology General Medicine DNA-binding domain Exons Sequence Analysis DNA Zinc finger nuclease Molecular biology Cell biology body regions DNA-Binding Proteins Repressor Proteins Animals Newborn Organ Specificity |
| Zdroj: | DNA and cell biology. 14(11) |
| ISSN: | 1044-5498 |
| Popis: | Using oligonucleotides complementary to the conserved inter-finger region of a variety of previously described zinc finger-encoding genes, a novel mouse gene was cloned and characterized. The gene is localized on chromosome 8 and comprises five exons. Its corresponding mRNA is developmentally regulated in various tissues and includes an open reading frame encoding a protein of 72,422 daltons. It shares amino-terminal homologies with human KRAB (or FPB) boxes, and contains 13 zinc fingers of the C2-H2 type. The NK10 KRAB domains exhibit repressing activity when tested in GAL4 fusion protein assays. Cloning of putative target sequences revealed that the individual domains differentially contribute to zinc-dependent target DNA binding. |
| Databáze: | OpenAIRE |
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