Patients with neoplastic and nonneoplastic hematologic diseases acquire CTLA-4 antibodies after blood transfusion
| Autor: | Toshihiro Matsui, Maria Pia Pistillo, Kusuki Nishioka, Carlo Gaudiano, Giovanni Battista Ferrara, Tomohiro Kato, Pier Luigi Tazzari, Vito Cilla, Paolo Capanni, Roberto Conte |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Hemolytic anemia Pathology medicine.medical_specialty Immunoconjugates Adolescent Recombinant Fusion Proteins Immunology Cold storage chemical and pharmacologic phenomena Immunofluorescence Antibodies Epitope Abatacept Immune system Antibody Specificity Antigens CD Humans Immunology and Allergy Medicine Blood Transfusion CTLA-4 Antigen Aged biology medicine.diagnostic_test business.industry Transfusion Reaction hemic and immune systems Hematology Middle Aged medicine.disease Antigens Differentiation Hematologic Diseases Leukemia CTLA-4 biology.protein Female Antibody business Epitope Mapping |
| Zdroj: | Transfusion. 41:462-469 |
| ISSN: | 1537-2995 0041-1132 |
| DOI: | 10.1046/j.1537-2995.2001.41040462.x |
| Popis: | BACKGROUND: The presence of antibodies to CTLA-4, a negative regulator of T-cell activation, was investigated in multiply transfused patients with malignant and non- malignant hematologic diseases. A previous study showed that, in multiply transfused patients, an immune response against nuclear matrix proteins can be induced by WBCs undergoing apoptosis during RBC unit storage. This study evaluated whether the same phenomenon could be involved in the induction of CTLA-4 antibodies in the patients analyzed. STUDY DESIGN AND METHODS: Patient sera were tested for binding to the recombinant full-length CTLA-4 β-galactosidase fusion protein by an ELISA. Immuno-fluorescence stainings were performed to analyze the CTLA-4 epitopes recognized by the antibodies and to detect such epitopes in the apoptotic cells present in the RBC units. RESULTS: CTLA-4 antibodies were found in multiply transfused patients with β-thalassemia (40%) and with other hemolytic diseases (33%) including leukemias (42%). A higher incidence of CTLA-4 antibodies was found in patients receiving non-WBC-reduced blood (88%) than in those receiving WBC-reduced blood (26%). Immunofluorescence staining showed that WBCs undergoing apoptosis in the RBC unit expressed CTLA-4 epitopes. CONCLUSIONS: The apoptotic WBCs present in the RBC units, after cold storage, express CTLA-4 epitopes. These epitopes can be released and induce formation of CTLA-4 antibodies with profound implications in the development of autoimmune disorders and in facilitating tumor dissemination and metastasis. |
| Databáze: | OpenAIRE |
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