A paracrine role for myoepithelial cell-derived FGF2 in the normal human breast

Autor: C L Johnston, R C Coope, C. Yiangou, J J Gomm, P. J. Browne, R. C. Coombes, R Mason, G. S. Bansal
Rok vydání: 1997
Předmět:
medicine.medical_specialty
Cell type
Basic fibroblast growth factor
Cell
Blotting
Western
Tetrazolium Salts
Biology
Fibroblast growth factor
Tritium
Epithelium
chemistry.chemical_compound
Paracrine signalling
Internal medicine
medicine
Humans
Breast
Receptor
Fibroblast Growth Factor
Type 2
Cells
Cultured
Chromatography
High Pressure Liquid
DNA Primers
Glucosamine
integumentary system
Fibroblast growth factor receptor 1
Myoepithelial cell
Receptor Protein-Tyrosine Kinases
Epithelial Cells
Cell Biology
Immunohistochemistry
Receptors
Fibroblast Growth Factor
Cell biology
Extracellular Matrix
Blotting
Southern
Endocrinology
medicine.anatomical_structure
chemistry
embryonic structures
Female
Fibroblast Growth Factor 2
biological phenomena
cell phenomena
and immunity
Mitogens
Cell Division
Subcellular Fractions
Zdroj: Experimental cell research. 234(1)
ISSN: 0014-4827
Popis: We have studied separated normal human breast epithelial and myoepithelial cells for the presence of basic fibroblast growth factor (FGF2) and its receptors, both low (heparan sulfate proteoglycans) and high affinity (FGFR1), and for the effects of FGF2 on the proliferation of both cell types. Our results indicate that these cells differ markedly in their synthesis and response to FGF2. We found, using PCR of purified cell populations, mRNA for FGF2 only in the myoepithelial cells, whereas immunostaining and Western blotting results demonstrated the presence of FGF2 protein in both epithelial and myoepithelial cells. FGF2 had no effect on the proliferation of myoepithelial cells, but it did maintain the survival of the separated epithelial cells in low serum and stimulate their growth in 5% and 10% FCS. Immunostainable FGFR1 was present in epithelial cells and, to a lesser extent, in myoepithelial cells. Low-affinity binding sites for FGF2 were synthesized by epithelial and myoepithelial cells, but myoepithelial cells possessed a greater proportion of higher-affinity heparan sulfate proteoglycans. These results indicate that myoepithelial cell-derived FGF2 may be an important paracrine factor controlling epithelial cell survival and growth in the normal human breast.
Databáze: OpenAIRE
Externí odkaz: