Identification of a nonsense mutation in feline ABCB1
| Autor: | Katrina L. Mealey, Neal S. Burke |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
ATP Binding Cassette Transporter
Subfamily B Nonsense mutation Biology Pharmacology Cat Diseases Polymorphism Single Nucleotide Praziquantel Neonicotinoids chemistry.chemical_compound Exon Central Nervous System Diseases Depsipeptides parasitic diseases medicine Animals Missense mutation Cloning Molecular Allele Ivermectin CATS General Veterinary Homozygote Imidazoles Exons Sequence Analysis DNA Nitro Compounds Molecular biology Moxidectin Selamectin chemistry Codon Nonsense Cats Emodepside Macrolides medicine.drug |
| Zdroj: | Journal of Veterinary Pharmacology and Therapeutics. 38:429-433 |
| ISSN: | 0140-7783 |
| DOI: | 10.1111/jvp.12212 |
| Popis: | The aim of this study was to sequence all exons of the ABCB1 (MDR1) gene in cats that had experienced adverse reactions to P-glycoprotein substrate drugs (phenotyped cats). Eight phenotyped cats were included in the study consisting of eight cats that experienced central nervous system toxicosis after receiving ivermectin (n = 2), a combination product containing moxidectin and imidacloprid (n = 3), a combination product containing praziquantel and emodepside (n = 1) or selamectin (n = 2), and 1 cat that received the product containing praziquantel and emodepside but did not experience toxicity (n = 1). Fifteen exons contained polymorphisms and twelve exons showed no variation from the reference sequence. The most significant finding was a nonsense mutation (ABCB11930_1931del TC) in one of the ivermectin-treated cats. This cat was homozygous for the deletion mutation. All of the other phenotyped cats were homozygous for the wild-type allele. However, 14 missense mutations were identified in one or more phenotyped cats. ABCB11930_1931del TC was also identified in four nonphenotyped cats (one homozygous and three heterozygous for the mutant allele). Cats affected by ABCB11930_1931del TC would be expected to have a similar phenotype as dogs with the previously characterized ABCB1-1Δ mutation. |
| Databáze: | OpenAIRE |
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