A Foodborne Outbreak of Gastroenteritis Associated with Norwalk‐like Viruses: First Molecular Traceback to Deli Sandwiches Contaminated during Preparation
| Autor: | Kate Hendricks, Stephan S. Monroe, Rebecca L. Fankhauser, Sudha Reddy, Nicholas A. Daniels, Steven M. Rowe, Roger I. Glass, David Bergmire-Sweat, Paul S. Mead, Robert L. Atmar, Kellogg J. Schwab |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Diarrhea Male Food handlers Adolescent Universities Food Handling Cafeteria Biology medicine.disease_cause Polymerase Chain Reaction Disease Outbreaks Foodborne Diseases Feces medicine Humans Immunology and Allergy Caliciviridae Infections Food poisoning digestive oral and skin physiology Infant Outbreak biology.organism_classification medicine.disease Texas Virology Caliciviridae Gastroenteritis Norwalk virus Infectious Diseases Case-Control Studies Norovirus Female Viral disease |
| Zdroj: | The Journal of Infectious Diseases. 181:1467-1470 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/315365 |
| Popis: | In March 1998, an outbreak of acute gastroenteritis occurred among students at a Texas university. Overall, 125 ill students sought medical care. Case-control studies revealed that illness was significantly associated with eating foods from the university's main cafeteria deli bar on 9 and 10 March. Stool specimens from 9 (50%) of 18 ill students and samples of deli ham showed evidence of Norwalk-like viruses (NLVs) by reverse-transcriptase (RT) polymerase chain reaction (PCR) assay. A food handler who prepared sandwiches for lunch on 9 March reported that her infant had been sick with watery diarrhea since just before the outbreak. A stool sample from the infant was positive for NLV by RT-PCR, and the sequence of the amplified product was identical to that of amplified product from deli ham and students' stool specimens. This is the first time RT-PCR and sequence analysis have successfully confirmed viral contamination of a food item likely to have been contaminated by a food handler. |
| Databáze: | OpenAIRE |
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