(D-ala, D-leu) enkephalin reduces the binding of GTP in hippocampal membranes
| Autor: | Shinno E, Reizo Inoki, Sadaaki Maeda, Tetsuo Ohnishi, Yasuhiro Ooi, Kihachi Saito, T. Aramaki |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Azides Indoles GTP' Enkephalin In Vitro Techniques Hippocampal formation Hippocampus General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley chemistry.chemical_compound GTP-Binding Proteins Naltrindole medicine Animals General Pharmacology Toxicology and Pharmaceutics Gel electrophoresis Membranes Photoaffinity labeling Affinity Labels General Medicine Enkephalin Leucine-2-Alanine Leu-enkephalin Naltrexone Rats Morphinans chemistry Biochemistry Guanosine 5'-O-(3-Thiotriphosphate) Biophysics Autoradiography Electrophoresis Polyacrylamide Gel DADLE Guanosine Triphosphate medicine.drug |
| Zdroj: | Life Sciences. 52:901-906 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(93)90524-7 |
| Popis: | The effect of (D-Ala, D-Leu) enkephalin (DADLE) on the binding of GTP in hippocampal preparations was studied. It was observed that treatment of hippocampal slices with 10 −5 −5×10 −5 M DADLE followed by the preparation of membrane fractions reduced the binding of 35 S-GTP-γ-S. There was no change in the affinity of the binding. This decrease of 35 S-GTP-γ-S binding was reversed when 5×10 −5 M naltrindole was included. The effect was not observed when the membrane fractions were incubated with DADLE. Photoaffinity labeling with the use of 32 P P3-(4-azidoanilido) -P1 5' -GTP followed by sodium dodecyl sulfate-polycrylamide gel electrophoresis and autoradiography revealed the incorporation of radioactivity into molecular mass of the 43 kDa and 33–34 kDa proteins. 32 P Photolabeling of both the 43 kDa and 33–34 kDa bands decreased following treatment of hippocampal slices with 10 −4 M DADLE. These results suggested that DADLE reduces the GDP-GTP exchange in hippocampal membranes. |
| Databáze: | OpenAIRE |
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