A new member of the ribbon-helix-helix transcription factor superfamily from the plant pathogen Xanthomonas axonopodis pv.citri
| Autor: | Maurizio Paci, Alberto Spisni, Chuck S. Farah, Elena Ferrari, Angela M. Katsuyama, Tommaso Eliseo, Thelma A. Pertinhez, Daniel O. Cicero, Irene Amata, Mariana Gallo |
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| Rok vydání: | 2009 |
| Předmět: |
Electrophoresis
Models Molecular Citrus Sequence analysis Protein Conformation Nuclear Magnetic Resonance Molecular Sequence Data Repressor Biology CANCRO (DOENÇA DE PLANTA) DNA-binding protein Fluorescence Microbiology Protein structure Structural Biology Models Amino Acid Sequence Settore BIO/10 Transcription factor Peptide sequence Gene Nuclear Magnetic Resonance Biomolecular Genetics Polyacrylamide Gel Base Sequence Spectrometry Molecular DNA Sequence Analysis DNA Spectrometry Fluorescence Citrus canker Xanthomonas axonopodis Electrophoresis Polyacrylamide Gel Plasmids Dimerization Transcription Factors Sequence Analysis Biomolecular |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1095-8657 |
| Popis: | XACb0070 is an uncharacterized protein coded by the two large plasmids isolated from Xanthomonas axonopodis pv. citri, the agent of citrus canker and responsible for important economical losses in citrus world production. XACb0070 presents sequence homology only with other hypothetical proteins belonging to plant pathogens, none of which have their structure determined. The NMR-derived solution structure reveals this protein is a homodimer in which each monomer presents two domains with different structural and dynamic properties: a folded N-terminal domain with beta alpha alpha topology which mediates dimerization and a long disordered C-terminal tail. The folded domain shows high structural similarity to the ribbon-helix-helix transcriptional repressors, a family of DNA-binding proteins of conserved 3D fold but low sequence homology: indeed XACb0070 binds DNA. Primary sequence and fold comparison of XACb0070 with other proteins of the ribbon-helix-helix family together with examination of the genes in the vicinity of xacb0070 suggest the protein might be the component of a toxin-antitoxin system. |
| Databáze: | OpenAIRE |
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