The role of nitric oxide radicals in removal of hyper-radiosensitivity by priming irradiation
| Autor: | Hilde S. Vollan, Agnes Görlach, Katharina Reger, Joe Alexander Sandvik, Nina Jeppesen Edin, Erik O. Pettersen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Free Radicals
DNA damage Health Toxicology and Mutagenesis Cell low dose-rate Priming (immunology) Nitric Oxide Synthase Type II Nitric Oxide Radiation Dosage Radiation Tolerance Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor medicine Humans Radiology Nuclear Medicine and imaging Irradiation Biology 030304 developmental biology 0303 health sciences Radiation biology Chemistry Radiochemistry Dose fractionation inducible nitric oxide synthase Dose-Response Relationship Radiation Molecular biology Adaptation Physiological Nitric oxide synthase medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis biology.protein Dose Fractionation Radiation hyper-radiosensitivity |
| Zdroj: | Journal of Radiation Research Journal of Radiation Research; Vol 54 |
| ISSN: | 1349-9157 0449-3060 |
| DOI: | 10.1093/jrr/rrt061 |
| Popis: | In this study, a mechanism in which low-dose hyper-radiosensitivity (HRS) is permanently removed, induced by low-dose-rate (LDR) (0.2–0.3 Gy/h for 1 h) but not by high-dose-rate priming (0.3 Gy at 40 Gy/h) was investigated. One HRS-negative cell line (NHIK 3025) and two HRS-positive cell lines (T-47D, T98G) were used. The effects of different pretreatments on HRS were investigated using the colony assay. Cell-based ELISA was used to measure nitric oxide synthase (NOS) levels, and microarray analysis to compare gene expression in primed and unprimed cells. The data show how permanent removal of HRS, previously found to be induced by LDR priming irradiation, can also be induced by addition of nitric oxide (NO)-donor DEANO combined with either high-dose-rate priming or exposure to prolonged cycling hypoxia followed by reoxygenation, a treatment not involving radiation. The removal of HRS appears not to involve DNA damage induced during priming irradiation as it was also induced by LDR irradiation of cell-conditioned medium without cells present. The permanent removal of HRS in LDR-primed cells was reversed by treatment with inducible nitric oxide synthase (iNOS) inhibitor 1400W. Furthermore, 1400W could also induce HRS in an HRS-negative cell line. The data suggest that LDR irradiation for 1 h, but not 15 min, activates iNOS, and also that sustained iNOS activation is necessary for the permanent removal of HRS by LDR priming. The data indicate that nitric oxide production is involved in the regulatory processes determining cellular responses to low-dose-rate irradiation. |
| Databáze: | OpenAIRE |
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