Embryonic origin and lineage hierarchies of the neural progenitor subtypes building the zebrafish adult midbrain

Autor: Lara Dirian, Sonya Galant, Marion Coolen, Laure Bally-Cuif, Giacomo Furlan, Isabelle Foucher
Přispěvatelé: Institut des Neurosciences Paris-Saclay (NeuroPSI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Régulation épigénétique - Epigenetic regulation, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Aging
Superior Colliculi
Optic tectum
Embryo
Nonmammalian
Neurogenesis
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Neuroepithelial Cells
Pallium
Radial glia
Biology
Article
03 medical and health sciences
0302 clinical medicine
Neural Stem Cells
Mesencephalon
medicine
Animals
Cell Lineage
Progenitor cell
Molecular Biology
Zebrafish
Progenitor
Neurons
Recombination
Genetic
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
Neural tube
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Cell Biology
Anatomy
biology.organism_classification
Embryonic stem cell
Neural stem cell
Neuroepithelial cell
Tamoxifen
030104 developmental biology
medicine.anatomical_structure
Doxycycline
Neuroepithelial progenitor
Neuroglia
Neuroscience
030217 neurology & neurosurgery
Developmental Biology
Post-embryonic neurogenesis
Zdroj: Developmental Biology
Developmental Biology, 2016, 420 (1), pp.120-135. ⟨10.1016/j.ydbio.2016.09.022⟩
Developmental Biology, Elsevier, 2016, 420 (1), pp.120-135. ⟨10.1016/j.ydbio.2016.09.022⟩
ISSN: 0012-1606
1095-564X
Popis: Neurogenesis in the post-embryonic vertebrate brain varies in extent and efficiency between species and brain territories. Distinct neurogenesis modes may account for this diversity, and several neural progenitor subtypes, radial glial cells (RG) and neuroepithelial progenitors (NE), have been identified in the adult zebrafish brain. The neurogenic sequences issued from these progenitors, and their contribution to brain construction, remain incompletely understood. Here we use genetic tracing techniques based on conditional Cre recombination and Tet-On neuronal birthdating to unravel the neurogenic sequence operating from NE progenitors in the zebrafish post-embryonic optic tectum. We reveal that a subpopulation of her5-positive NE cells of the posterior midbrain layer stands at the top of a neurogenic hierarchy involving, in order, the amplification pool of the tectal proliferation zone (TPZ), followed by her4-positive RG cells with transient neurogenic activity. We further demonstrate that the adult her5-positive NE pool is issued in lineage from an identically located NE pool expressing the same gene in the embryonic neural tube. Finally, we show that these features are reminiscent of the neurogenic sequence and embryonic origin of the her9-positive progenitor NE pool involved in the construction of the lateral pallium at post-embryonic stages. Together, our results highlight the shared recruitment of an identical neurogenic strategy by two remote brain territories, where long-lasting NE pools serve both as a growth zone and as the life-long source of young neurogenic RG cells.
Highlights • Zebrafish post-embryonic tectal neurogenesis is driven by neuroepithelial progenitors. • The neuroepithelial progenitor pool is long-lasting and expresses Her5 life long. • Tectal radial glia originate from the her5-positive pool and are transiently neurogenic. • The post-embryonic neurogenic sequences of the tectum and lateral pallium are similar.
Databáze: OpenAIRE
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