LncRNA-H19 activates CDC42/PAK1 pathway to promote cell proliferation, migration and invasion by targeting miR-15b in hepatocellular carcinoma
| Autor: | Xu-Dong Wu, Wen-Zhang Zha, Jing Jia, Yong Zhou, Ren-Gen Fan, Cheng-Lin Qin |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Adult Male Carcinoma Hepatocellular CDC42 Biology 01 natural sciences Flow cytometry 03 medical and health sciences PAK1 Cell Movement Genetics medicine Humans Neoplasm Invasiveness RNA Neoplasm cdc42 GTP-Binding Protein 030304 developmental biology Aged Cell Proliferation 0303 health sciences Gene knockdown medicine.diagnostic_test Cell growth Liver Neoplasms Hep G2 Cells Middle Aged female genital diseases and pregnancy complications Neoplasm Proteins Blot MicroRNAs p21-Activated Kinases Apoptosis embryonic structures Cancer research Immunohistochemistry Female RNA Long Noncoding 010606 plant biology & botany |
| Zdroj: | Genomics. 111(6) |
| ISSN: | 1089-8646 |
| Popis: | Background Hepatocellular carcinoma (HCC) is one of the main causes of cancer-related death. This study aims to explore the role and underlying mechanism of H19 in HCC. Methods qRT-PCR detected miR-15b-5p and H19 expression, as well as the mRNA level of EMT-associated genes. Western blotting detected protein level of EMT-associated genes. Immunohistochemistry (IHC) examined CDC42 in HCC tissues. Dual luciferase reporter assay verified the regulatory mechanism among H19, miR-15b and CDC42. Colony formation, wound healing assay, transwell, flow cytometry measured proliferation, migration, invasion and apoptosis, respectively. Results H19 and CDC42 were up-regulated while miR-15b was down-regulated in HCC cells and tissues. miR-15b interacted with H19 and CDC42 3′-UTR. H19 knockdown inhibited proliferation, migration and invasion, and increased apoptosis, which was rescued by miR-15b inhibitor. H19 knockdown suppressed CDC42/PAK1 pathway and EMT progress. Conclusion H19 knockdown inhibited proliferation, migration and invasion, and promoted apoptosis of HCC cells via targeting miR-15b/CDC42/PAK1 axis. |
| Databáze: | OpenAIRE |
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