Hydrogen Peroxide from the Oxidative Burst Is Neither Necessary Nor Sufficient for Hypersensitive Cell Death Induction, Phenylalanine Ammonia Lyase Stimulation, Salicylic Acid Accumulation, or Scopoletin Consumption in Cultured Tobacco Cells Treated with Elicitin
Autor: | Stéphan Dorey, Pierrette Geoffroy, Bernard Fritig, Marguerite Kopp, Serge Kauffmann |
---|---|
Rok vydání: | 1999 |
Předmět: |
Phytophthora
Hypersensitive response Physiology Nicotiana tabacum Plant Science Phenylalanine ammonia-lyase Biology chemistry.chemical_compound Plant Growth Regulators Scopoletin Tobacco Genetics Cells Cultured Glycoproteins Phenylalanine Ammonia-Lyase Plant Diseases Respiratory Burst Cell Death Algal Proteins food and beverages Elicitin Hydrogen Peroxide biology.organism_classification Respiratory burst Elicitor Enzyme Activation Plant Leaves Plants Toxic chemistry Biochemistry Salicylic Acid Salicylic acid Research Article |
Zdroj: | Plant Physiology. 121:163-172 |
ISSN: | 1532-2548 0032-0889 |
DOI: | 10.1104/pp.121.1.163 |
Popis: | H2O2 from the oxidative burst, cell death, and defense responses such as the production of phenylalanine ammonia lyase (PAL), salicylic acid (SA), and scopoletin were analyzed in cultured tobacco (Nicotiana tabacum) cells treated with three proteinaceous elicitors: two elicitins (α-megaspermin and β-megaspermin) and one glycoprotein. These three proteins have been isolated from Phytophthora megasperma H20 and have been previously shown to be equally efficient in inducing a hypersensitive response (HR) upon infiltration into tobacco leaves. However, in cultured tobacco cells these elicitors exhibited strikingly different biological activities. β-Megaspermin was the only elicitor that caused cell death and induced a strong, biphasic H2O2 burst. Both elicitins stimulated PAL activity similarly and strongly, while the glycoprotein caused only a slight increase. Only elicitins induced SA accumulation and scopoletin consumption, and β-megaspermin was more efficient. To assess the role of H2O2 in HR cell death and defense response expression in elicitin-treated cells, a gain and loss of function strategy was used. Our results indicated that H2O2 was neither necessary nor sufficient for HR cell death, PAL activation, or SA accumulation, and that extracellular H2O2 was not a direct cause of intracellular scopoletin consumption. |
Databáze: | OpenAIRE |
Externí odkaz: |