MiR-3120 is a mirror microRNA that targets heat shock cognate protein 70 and auxilin messenger RNAs and regulates clathrin vesicle uncoating
| Autor: | Youn-Bok Lee, Helen L. Scott, Leonidas A. Phylactou, Liang-Fong Wong, Paul Verkade, James B. Uney, Ioannis Bantounas, Joanna Howarth |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Auxilins
Auxilin Biology Heat Shock Protein Biochemistry Clathrin 03 medical and health sciences 0302 clinical medicine Neurobiology Gene expression microRNA Vesicle uncoating Gene silencing Animals HSP70 Heat-Shock Proteins RNA Messenger Vesicles Rats Wistar Molecular Biology 030304 developmental biology Regulation of gene expression Neurons 0303 health sciences RNA Clathrin-Coated Vesicles MicroRNA Cell Biology Molecular biology Endocytosis Rats MicroRNAs Gene Expression Regulation biology.protein Dynamin III 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Biological Chemistry |
| ISSN: | 1083-351X |
| Popis: | Background: “Mirrored” or complementary mammalian miRNAs have been predicted but none have yet been characterized. Results: miR-3120 and miR-214 are produced from the same intronic locus; miR-3120 regulates heat shock cognate protein 70 (Hsc70) and auxilin expression and vesicle uncoating. Conclusion: miR-3120 is a mirror miRNA regulating endocytic function. Significance: Mirror miRNAs are important mammalian gene regulatory units, and many more remain to be found and characterized. We show that a single gene locus gives rise to two fully processed and functional miRNAs, i.e. that due to imperfect base pairing, two distinct microRNAs (miRNAs) can be produced from the fully complementary DNA strands. The antisense strand encodes miR-214, which is transcribed by its own promoter, whereas a novel miRNA, miR-3120, is co-expressed with its host gene mRNA. We also found that miR-3120 regulates important aspects of cellular function that are similar to that of its host gene, dynamin-3. miR-3120 was found to be located in neuronal cell bodies and to target Hsc70 and auxilin, and its lentivirus-mediated expression inhibited the uncoating of clathrin-coated vesicles. Finally, mirror miRNAs are likely to represent a new group of miRNAs with complex roles in coordinating gene expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|