Myocardial energy metabolism and morphology in a canine model of sepsis
| Autor: | M. A. Solomon, R. Correa, H. R. Alexander, L. A. Koev, J. P. Cobb, D. K. Kim, W. C. Roberts, Z. M. Quezado, T. D. Scholz, R. E. Cunnion, al. et |
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| Rok vydání: | 1994 |
| Předmět: |
Cardiac function curve
medicine.medical_specialty Pathology Time Factors Endothelium Epinephrine Phosphocreatine Physiology Bacteremia Fibrin Mitochondria Heart Ventricular Function Left Sepsis Phenylephrine Adenosine Triphosphate Dogs Oxygen Consumption Myofibrils Reference Values Physiology (medical) Internal medicine Carnivora medicine Animals Escherichia coli Infections Ejection fraction biology Myocardium Fissipedia Heart medicine.disease biology.organism_classification Disease Models Animal medicine.anatomical_structure Rate pressure product Endocrinology biology.protein Endothelium Vascular Cardiology and Cardiovascular Medicine Energy Metabolism Mitochondrial Swelling |
| Zdroj: | The American journal of physiology. 266(2 Pt 2) |
| ISSN: | 0002-9513 |
| Popis: | The mechanism responsible for sepsis-induced myocardial depression is not known. To determine if sepsis-induced myocardial depression is caused by inadequate free energy available for work, we studied myocardial energy metabolism in a canine model of sepsis. Escherichia coli-infected (n = 18) or sterile (n = 16) fibrin clots were implanted intraperitoneally into beagles. Myocardial function and structure was assessed using radionuclide ventriculograms, echocardiograms, and light and electron microscopy. The adequacy of energy metabolism was evaluated by comparing catecholamine-induced work increases [myocardial O2 consumption (MVO2) and rate pressure product (RPP)] with a simultaneously obtained estimate of intracellular free energy [phosphocreatine-to-adenosine triphosphate ratio (PCr:ATP)] determined by 31P-magnetic resonance spectroscopy. When compared with control animals, septic animals had a decrease in left ventricular ejection fraction (EF, P < 0.0001) on day 1 and fractional shortening (FS, P < 0.0003) on day 2 after clot implantation. On day 2, neither septic nor control animals had statistically significant decreases in PCr:ATP, despite catecholamine-induced increases in MVO2 and RPP (mean maximal increases in septic animals 135 +/- 31 and 51 +/- 10%, respectively). Light and electron microscopic findings showed that hearts of septic animals, compared with control animals, had a greater degree of morphological abnormalities. Thus, in a canine model of sepsis with alterations in myocyte ultrastructure and documented myocardial depression (decreased EF and FS), intracellular free energy levels (PCr:ATP) were maintained despite catecholamine-induced increases in myocardial work (increased MVO2 and RPP), suggesting high-energy synthetic capabilities are not limiting cardiac function. |
| Databáze: | OpenAIRE |
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