Analysis of genetic variation in mitochondrial cytochrome c oxidase subunit 1 between Haemadipsa japonica in Japan and land leeches worldwide
| Autor: | Keito Nagase, Takafumi Nakano, Katsuya Iuchi, Saeko Miyashita, Miki Inukai, Naoe Sato, Chikako Yokoyama, Hisashi Hisatomi |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine the maximum likelihood method (ML) tree Oxidase test Mitochondrial DNA Range (biology) Protein subunit Haemadipsa japonica food and beverages Leech Zoology macromolecular substances Biology 010603 evolutionary biology 01 natural sciences Mitochondrial cox1 03 medical and health sciences 030104 developmental biology parasitic diseases Genetic variation Genetics Mitochondrial cytochrome Molecular Biology |
| Zdroj: | Mitochondrial DNA Part B. 4:1408-1410 |
| ISSN: | 2380-2359 |
| Popis: | Haemadipsa japonica is the most common land leech species found in Japan. It has been considered to possess genetic variation that limits its habitat range. In the present study, to characterize variation in the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of H. japonica specimens from various locations in Japan, we examined nucleotide sequences of the mitochondrial cox1 gene. We performed PCR of mitochondrial cox1 using 10 H. japonica specimens and compared the result with those of land leeches from around the world using a maximum likelihood (ML) tree. ML tree of H. japonica in Japan showed significant differences between cox1 sequences of specimens from Yakushima and other regions of Japan. ML tree of land leeches from around the world revealed that H. japonica had the closest relationship with H. picta from Borneo. |
| Databáze: | OpenAIRE |
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