Comparative proteomics of two Mycoplasma hyopneumoniae strains and Mycoplasma flocculare identified potential porcine enzootic pneumonia determinants

Autor:	Fernanda Munhoz dos Anjos Leal, Hercules Moura, Lais Del Prá Netto Machado, Jéssica Andrade Paes, Henrique Bunselmeyer Ferreira, John R. Barr, Sofia Nóbrega De Moraes
Rok vydání:	2018
Předmět:	0301 basic medicine Microbiology (medical) Proteome Swine 030106 microbiology Immunology virulence factors Virulence subcellular fractions medicine.disease_cause Proteomics Microbiology Mass Spectrometry lcsh:Infectious and parasitic diseases 03 medical and health sciences Porcine enzootic pneumonia Bacterial Proteins Species Specificity Mycoplasma hyopneumoniae medicine Animals lcsh:RC109-216 Adhesins Bacterial mycoplasma swine respiratory pathogens bacterium-host interactions Host Microbial Interactions biology Mycoplasma Pneumonia of Swine Mycoplasmal biology.organism_classification 030104 developmental biology Infectious Diseases medicine.anatomical_structure Parasitology Comparative proteomics Mycoplasma flocculare Bacteria Research Paper Peptide Hydrolases Respiratory tract
Zdroj:	Virulence, Vol 9, Iss 1, Pp 1230-1246 (2018) Virulence
ISSN:	2150-5608 2150-5594
DOI:	10.1080/21505594.2018.1499379
Popis:	Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar bacteria, which coinhabit the porcine respiratory tract. These mycoplasmas share most of the known virulence factors, but, while M. hyopneumoniae causes porcine enzootic pneumonia (PEP), M. flocculare is a commensal species. To identify potential PEP determinants and provide novel insights on mycoplasma-host interactions, the whole cell proteomes of two M. hyopneumoniae strains, one pathogenic (7448) and other non-pathogenic (J), and M. flocculare were compared. A cell fractioning approach combined with mass spectrometry (LC-MS/MS) proteomics was used to analyze cytoplasmic and surface-enriched protein fractions. Average detection of ~ 50% of the predicted proteomes of M. hyopneumoniae 7448 and J, and M. flocculare was achieved. Many of the identified proteins were differentially represented in M. hyopneumoniae 7448 in comparison to M. hyopneumoniae J and M. flocculare, including potential PEP determinants, such as adhesins, proteases, and redox-balancing proteins, among others. The LC-MS/MS data also provided experimental validation for several genes previously regarded as hypothetical for all analyzed mycoplasmas, including some coding for proteins bearing virulence-related functional domains. The comprehensive proteome profiling of two M. hyopneumoniae strains and M. flocculare provided tens of novel candidates to PEP determinants or virulence factors, beyond those classically described.
Databáze:	OpenAIRE
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