A simple time-resolved fluorescence assay for quantitative determination of DOTA chelator
| Autor: | Magdalena Janczewska, Kamil Brygoła, Michał Szkop, Tomasz Ciach |
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| Rok vydání: | 2019 |
| Předmět: |
Time Factors
Biophysics chemistry.chemical_element 01 natural sciences Biochemistry Metal Chelator Heterocyclic Compounds 1-Ring 03 medical and health sciences chemistry.chemical_compound DOTA Chelation Molecular Biology Chelating Agents 030304 developmental biology 0303 health sciences Quenching (fluorescence) Chromatography Chemistry 010401 analytical chemistry Cell Biology 0104 chemical sciences Spectrometry Fluorescence Reagent Calibration Time-resolved spectroscopy Europium Luminescence |
| Zdroj: | Analytical Biochemistry. 584:113384 |
| ISSN: | 0003-2697 |
| Popis: | DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate) is one of the preeminent metal chelator applied for diagnostic and therapeutic purposes, however to date there is no versatile and reliable nonradioisotopic method for its precise determination. In this technical note, we present a novel and sensitive fluorimetric assay for quantitative determination of DOTA based on the luminescence quenching of the highly luminescent europium ions complex with trioctyl phosphine oxide and naphthoyl trifluoroacetone sensitizing activators. The assay is carried out in two simple steps and enables the determination of DOTA in the nanomolar range providing a superior tool compared to commonly applied spectrophotometric assay with Arsenazo-III reagent. |
| Databáze: | OpenAIRE |
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