Numerical abberations of chromosomes 1 and 7 in renal cell carcinomas as detected by interphase cytogenetics

Autor: P.C.M. de Wilde, J. L. M. Beck, Wout Feitz, H. E. Schaafsma, Antonius G.J.M. Hanselaar, C.A. Hulsbergen van de Kaa, Frans C. S. Ramaekers, Anton H. N. Hopman, J.A. Schalken
Rok vydání: 1995
Předmět:
Monosomy
Identification and characterization of molecular parameters with predictive value for the progression of superficial bladder cancer
Biology
urologic and male genital diseases
Pathology and Forensic Medicine
Cytogenetisch patroon van de tumor progressie in de intraepitheliale neoplasie van de cervix uteri
Centromere
medicine
Humans
Identificatie en karakterisering van moleculaire parameters met voorspellende waarde voor de progressie van het oppervlakkige blaascarcinoom
GeneralLiterature_REFERENCE(e.g.
dictionaries
encyclopedias
glossaries)
Carcinoma
Renal Cell
Interphase
In Situ Hybridization
Fluorescence
Chromosome 7 (human)
Chromosome Aberrations
medicine.diagnostic_test
Hybridization probe
Chromosome
Karyotype
medicine.disease
Flow Cytometry
Molecular biology
Cytogenetic pattern of tumor progression in uterine cervical intraepithelial neoplasia
Kidney Neoplasms
Chromosomes
Human
Pair 1
Ploidy
Chromosomes
Human
Pair 7
Fluorescence in situ hybridization
Zdroj: Journal of Pathology, 176, 123-125
Journal of Pathology, 176, 2, pp. 123-125
Journal of Pathology, 176, 123-135
Journal of Pathology, 176, pp. 123-135
Journal of Pathology, 176, pp. 123-125
ISSN: 0022-3417
Popis: Alcohol-fixed single cell suspensions of 37 renal cell carcinomas (RCCs) were assessed by both flow cytometry (FCM) and the fluorescence in situ hybridization (FISH) technique, using chromosome 1- and chromosome 7-specific centromere DNA probes. DNA diploidy or near-diploidy was observed in 30 of the 37 RCCs and only 12 of these (near-)diploid tumours were disomic for both chromosomes 1 and 7. Numerical aberrations of chromosome 1 and/or chromosome 7 were present in 18 of the 30 (near-)diploid RCCs and five of these cases showed monosomy for chromosome 1 in more than 50 per cent of the tumour cells. A double target FISH, with a centromeric and a telomeric specific probe for 1p36, excluded misinterpretation on the basis of clustering of 1q12, and suggested a complete loss of chromosome 1. All these five (near-)diploid RCCs with monosomy for chromosome 1 were eosinophilic chromophilic cell carcinomas, according to the Thoenes classification of RCC. This observation is of special interest, because it was recently concluded from cytogenetic studies that the diagnosis of chromophilic renal cell carcinoma must be considered as obsolete. Monosomy for chromosome 1 seems to be a non-random numerical aberration of (near-)diploid eosinophilic chromophilic cell carcinomas, and a gain of one or more chromosomes 1 appeared to be a common phenomenon in RCCs, especially in the DNA aneuploid tumours. As these chromosomal abnormalities were not found in the earlier classical cytogenetic studies, we conclude that in situ hybridization techniques are required in addition to chromosome banding techniques to obtain a complete characterization of the chromosome imbalances in RCCs.
Databáze: OpenAIRE
Externí odkaz: