Heat shock protein 27 protects against aminolevulinic acid-mediated photodynamic therapy-induced apoptosis and necrosis in human breast cancer cells
| Autor: | Steen J. Madsen, Stephen W. Carper, Sarah A. Ziegler, Cherisse R. Loucks |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Necrosis Cell Survival Health Toxicology and Mutagenesis medicine.medical_treatment Photodynamic therapy Apoptosis Breast Neoplasms Toxicology Transfection Pathology and Forensic Medicine chemistry.chemical_compound Hsp27 Heat shock protein medicine Tumor Cells Cultured Humans Propidium iodide Heat-Shock Proteins Photosensitizing Agents biology Chemistry Dose-Response Relationship Radiation General Medicine Aminolevulinic Acid Molecular biology Surgery Photochemotherapy Cell culture Cancer cell biology.protein medicine.symptom |
| Zdroj: | Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer. 26(3) |
| ISSN: | 0731-8898 |
| Popis: | This study utilized two breast cancer cell lines differing only in their expression of heat shock protein 27 (hsp27). The DB46 cell line was engineered to express high constitutive levels of hsp27, while the DC4 cell line expresses normal low levels of hsp27. The cells were incubated in 1 mM aminolevlinic acid (ALA) 4 hr prior to light exposures (635 nm) ranging from 1 to 20 J/cm2. Both cell lines displayed a dose response to photodynamic therapy (PDT) as assayed by clonogenic survival. LD50s of 2.68 and 1.27 J/cm2 were observed for DB46 and DC4 cells respectively. ALA-PDT-induced resistance to both apoptosis and necrosis in the DB46 cell line was found from TUNEL assays and fluorescence microscopy studies using propidium iodide and Hoechst staining. |
| Databáze: | OpenAIRE |
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