A Murine Oral‐Exposure Model for Nano‐ and Micro‐Particulates: Demonstrating Human Relevance with Food‐Grade Titanium Dioxide
| Autor: | John W. Wills, Harjinder Singh, Stuart Micklethwaite, Andy Brown, Don Otter, Michelle Miniter, Ravin Jugdaohsingh, Nicole C. Roy, Paul Rees, Jonathan J. Powell, Rachel E. Hewitt, Sebastian Riedle |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Food intake
Physiology Administration Oral Metal Nanoparticles 02 engineering and technology 010402 general chemistry Weight Gain 01 natural sciences Airborne particle Risk Assessment Biomaterials chemistry.chemical_compound Eating Mice Peyer's Patches Immune system Oral route medicine Animals Humans General Materials Science Dosing Titanium Food grade General Chemistry Environmental Exposure 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Titanium dioxide Models Animal medicine.symptom 0210 nano-technology Weight gain Biotechnology |
| Popis: | Human exposure to persistent, nonbiological nanoparticles and microparticles via the oral route is continuous and large scale (1012 -1013 particles per day per adult in Europe). Whether this matters or not is unknown but confirmed health risks with airborne particle exposure warns against complacency. Murine models of oral exposure will help to identify risk but, to date, lack validation or relevance to humans. This work addresses that gap. It reports i) on a murine diet, modified with differing concentrations of the common dietary particle, food grade titanium dioxide (fgTiO2 ), an additive of polydisperse form that contains micro- and nano-particles, ii) that these diets deliver particles to basal cells of intestinal lymphoid follicles, exactly as is reported as a "normal occurrence" in humans, iii) that confocal reflectance microscopy is the method of analytical choice to determine this, and iv) that food intake, weight gain, and Peyer's patch immune cell profiles, up to 18 weeks of feeding, do not differ between fgTiO2 -fed groups or controls. These findings afford a human-relevant and validated oral dosing protocol for fgTiO2 risk assessment as well as provide a generalized platform for application to oral exposure studies with nano- and micro-particles. |
| Databáze: | OpenAIRE |
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