Cardiac stem cell transplantation with 2,3,5,4′-tetrahydroxystilbehe-2-O-β-d-glucoside improves cardiac function in rat myocardial infarction model
Autor: | Xuanxuan Zhou, Fan Song, Yanhua Xie, Jiyuan Sun, Weixun Duan, Hua Li, Li Yan, Siwang Wang, Qian Yang, Fei Hua |
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Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine Cardiac function curve medicine.medical_specialty Myocardial Infarction Connexin General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Glucosides Heart Rate In vivo Internal medicine Stilbenes Heart rate medicine Animals Myocytes Cardiac Myocardial infarction General Pharmacology Toxicology and Pharmaceutics Ejection fraction business.industry General Medicine medicine.disease Rats Transplantation Disease Models Animal 030104 developmental biology 030220 oncology & carcinogenesis Cardiology Stem cell business Stem Cell Transplantation |
Zdroj: | Life Sciences. 158:37-45 |
ISSN: | 0024-3205 |
Popis: | Headings aims Cardiac stem cells (CSCs)-transplanted therapy provides a promising therapy for the ischemic heart disease (IHD), especially in the epidemic of myocardial infarction (MI). The compound 2,3,5,4′-tetrahydroxystilbene-2-O-β- d -glucoside (THSG) can induce CSC proliferation in vitro based on our previous study, so we aimed to study the induce effect of THSG on CSCs-transplanted MI rat in vivo. Materials and methods Using a murine model of MI, this study was designed to evaluate the impact of THSG (30, 60, 120 mg/kg) on CSCs-based therapy for MI and the underlying mechanism in this process. Key finding The results showed that THSG on CSCs-transplanted therapy groups (THSG + CSCs groups) can significantly reduce S-T segment elevation, and increase heart rate compared with MI group. The left ventricular ejection fraction (LVEF) and the left ventricular fractional shortening (LVFS) were significantly reduced in THSG + CSCs groups compared to the MI group. The levels of enzyme expression (CK-MB, LDH), the heart weight index (HWI) and myocardial infarct size (IS) were all reduced in THSG + CSCs groups. Moreover, other changes noted during these 28 days post-MI, included pathologic changes, as well as increased stem cell antigen-1 (Sca-1) expression, or expression of Nkx2.5, GATA-4, and Connexin 43 in myocardial tissue, and reduced the Caspase-3 expression. Significance Our findings indicated that THSG facilitated CSCs-transplanted therapy in MI. These observations may be associated with the inducted of THSG on the proliferation of CSCs in vivo and also, with the subsequent differentiation of additional intrinsic neonatal cardiomyocytes to replace damaged heart tissue. |
Databáze: | OpenAIRE |
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