A two-site monoclonal antibody immunoradiometric assay for human follistatin: secretion by a human ovarian teratocarcinoma-derived cell line (PA-1)

Autor: A. R. Midgley, Patrick M. Sluss, D S McConnell, Qifa Wang, Alan L. Schneyer, V Padmanahban, R H Khoury, P C Smith, William F. Crowley
Rok vydání: 1996
Předmět:
Male
Teratocarcinoma
Follistatin
Endocrinology
Diabetes and Metabolism
Clinical Biochemistry
Biochemistry
Rats
Sprague-Dawley
Endocrinology
Antibody Specificity
Reference Values
Tumor Cells
Cultured
Cells
Cultured
Ovarian Neoplasms
Immunoradiometric assay
biology
Antibodies
Monoclonal
Recombinant Proteins
Activins
Blot
Pituitary Gland
embryonic structures
Biological Assay
Female
Immunoradiometric Assay
Antibody
hormones
hormone substitutes
and hormone antagonists
Cell Division
endocrine system
medicine.medical_specialty
medicine.drug_class
Blotting
Western
Cross Reactions
Monoclonal antibody
Sensitivity and Specificity
Cell Line
Corpus Luteum
Pituitary Gland
Anterior
Internal medicine
medicine
Animals
Humans
Secretion
Inhibins
Autocrine signalling
Glycoproteins
Granulosa Cells
Biochemistry (medical)
Reproducibility of Results
Follicular Fluid
Rats
Molecular Weight
Cell culture
Culture Media
Conditioned
biology.protein
Zdroj: The Journal of clinical endocrinology and metabolism. 81(4)
ISSN: 0021-972X
Popis: The follistatin/activin/inhibin system increasingly appears to have important growth and differentiating effects in a variety of cell types, including cancer. We have developed a two-site immunoradiometric assay for measurement of human follistatin using two monoclonal antibodies against recombinant human follistatin. This cloned protein donor assay is sensitive (0.5 ng/mL), specific for free human follistatin, and precise (
Databáze: OpenAIRE
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