| Autor: |
Hervás-Salcedo, Rosario, Fernández-García, María, Hernando-Rodríguez, Miriam, Quintana-Bustamante, Oscar, Jose-Carlos Segovia, Alvarez-Silva, Marcio, García-Arranz, Mariano, Minguez, Pablo, Pozo, Victoria Del, Alba, Marta Rodríguez De, García-Olmo, Damián, Ayuso, Carmen, Lamana, María Luisa, Bueren, Juan A., Yañez, Rosa María |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.13978726 |
| Popis: |
Additional file 2: Figure S2. Optimization of mRNA transfection in human Ad-MSCs. (A) Influence of different lipofectamines upon the transfection of Ad-MSCs with a control mRNA encoding for the EGFP marker protein. Transfection efficacy was analyzed by flow cytometry 24 h after transfection. (B) Influence of the mRNA concentration upon the transfection of Ad-MSCs. Analyses were performed as in panel A. (C) Analysis of human CXCR4 expression in Ad-MSCs by flow cytometry 24 h after transfection with 1 μg of CXCR4-mRNA and CXCR4-IL10 mRNA using different lipofectamine concentrations. (D) Human IL10 secretion of Ad-MSCs transfected with 1 μg of IL10-mRNA and CXCR4-IL10 mRNA. Analyses were performed by ELISA in supernatants collected 24h after transfection of the different mRNAs using different concentrations of lipofectamine. (E) Viability of Ad-MSCs analyzed at different times after transfection with the different mRNAs, using the ATP luminescence assay (see Materials and Methods). Data represents the mean ± SD of at least n = 3 different experiments. Statistical differences between mRNA-transfected MSCs and WT-MSCs were calculated with Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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